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270025 UE Opt. Pract. Exercises: Proteinanalytics (2021W)

3.00 ECTS (3.00 SWS), SPL 27 - Chemie
Continuous assessment of course work
MIXED

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

1. Kurs: 04.10.-08.10.2021
2. Kurs: 11.10.-15.10.2021
Die Anwesenheit am 04.10.2021, 10-12, bei der ersten Einheit des Praktikums (vor Ort im SR3) zu Vorbesprechung, Einteilung, sowie Theorieteil I ist für die Teilnehmer*innen beider Kurse verpflichtend. Generell muss die Anwesenheit während des Praktikums zu 90% erfüllt sein. Theorieteil II für die Teilnehmer*innen beider Kurse findet am 07.10.2021, 13-15h, ebenfalls im SR3 statt.

Monday 04.10. 10:00 - 12:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1
Thursday 07.10. 13:00 - 15:00 Seminarraum 3 Organische Chemie 1OG Boltzmanngasse 1

Information

Aims, contents and method of the course

The proteome of a complex sample shall be analyzed. Therefore, first, the complexity of the sample will be reduced using appropriate separation techniques. After that, an analysis of the sample, applyig mass spectrometry, will be performed. In the course of this practical work, different methods for protein analysis will be presented and practically applied.

Assessment and permitted materials

Performance is monitored during the practical course (interest, cooperation), and furthermore determined on the basis of the quality of the delivered protocol and the acquired knowledge (determined in the closing meeting).

Minimum requirements and assessment criteria

Students need positive grades for each part of the performance (interest/cooperation, protocol and closing meeting regarding the acquired knowledge) in order to successfully complete the course.

Examination topics

Background knowledge on the conducted experiments: preparation of biological samples, cell disruption, subcellular fractionation (separation of cytoplasmic and nuclear proteins), protein separation techniques using 2D- and 1D-gel electrophoresis, digestion of proteins into petides, identification of proteins using LC-MS/MS analyses.

Reading list

Additional literature is not required.

Association in the course directory

AN-1, AN-2, BC-1, CHE II-1

Last modified: Fr 01.10.2021 11:32