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270028 VO Semisynthesis of Proteins (2020S)

Methods for site-specific protein modifications by chemical and molecular biolobigy approaches

3.00 ECTS (2.00 SWS), SPL 27 - Chemie

Registration/Deregistration

Details

Language: German

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

Beginn der Vorlesung am Freitag, 13. März, 09.15 Uhr, Seminarraum 1.
Vorlesungszeiten: Freitag, 09.15 - 10.45 Uhr, Seminarraum 1, 2. Stock, Währinger Str. 38.

Friday 13.03. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 20.03. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 27.03. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 03.04. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 24.04. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 08.05. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 15.05. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 22.05. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 05.06. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 12.06. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 19.06. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1
Friday 26.06. 09:15 - 10:45 Seminarraum 1 Analytische Chemie 2.OG Boltzmanngasse 1

Information

Aims, contents and method of the course

Informationen zur Vorlesung:
http://www.univie.ac.at/biologischechemie1/semisynthese/

Auxotrophie-Stämme: globaler Einbau unnatürlicher Aminosäuren, Selenomethionin

In vitro Suppression: Amber-Codon, chemisch beladene Suppressor-tRNAs, Ortsspezifität

In vivo Suppression: Orthogonales tRNA/ AaRS-Paar, Proteinevolution

Chemische Modifikation von Proteinen: Biokonjugation der proteinogenen Aminosäuren (Lys, Cys),
Oxidation N-Terminus, PLP-vermittelte Ligation, Cross-Linker

Fluoreszierende Proteine: GFP und weitere Beispiele

Chemoselektive Ligationsreaktionen: Biokonjugation artifizieller Gruppen, Oxim-Ligation, Cu(I)-katalysierte 1,3-dipolare Cycloaddition, Staudinger-Ligation, Modifikation an lebenden Zellen

Chemische Peptid-/ Proteinligation: Peptidfestphasensynthese, Sekundärstrukturbrecher, Peptidsegmentkopplung, Native Chemische Ligation, Cys-freie Ligation, Auxiliare, Inteine, Expressed Protein Ligation

Enzymatische Peptidligation: Reverse Proteolyse, Sortase, gespaltene Inteine

Assessment and permitted materials

Exam

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

BC-3, CHE II-3, CHE II-4

Last modified: Th 26.11.2020 16:08