270041 UE Opt. Pract. Exercises: Proteinanalytics (2022S)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Tu 01.02.2022 08:00 to Th 24.02.2022 23:59
- Deregistration possible until Th 24.02.2022 23:59
Details
max. 12 participants
Language: German
Lecturers
Classes
1. Kurs: 25. - 29. 04. 2022; tägl. zw. 9h & spätestens 18h (6 Teilnehmer*innen)
2. Kurs: 02. - 06. 05. 2022; tägl. zw. 9h & spätestens 18h (6 Teilnehmer*innen)
Die Anwesenheit am 25. 04. 2022 zw. 9h00 & 11h00, bei der ersten Einheit des Praktikums zu Vorbesprechung, Einteilung, sowie Theorieteil I im HS 3, ist für die Teilnehmer*innen beider Kurse verpflichtend.
Generell muss die Anwesenheit während des Praktikums zu 90% erfüllt sein. Theorieteil II, ebenfalls für die Teilnehmer*innen beider Kurse, findet am 28. 04. 2022 im HS 4 zw. 13h00 & 15h00 statt.
Information
Aims, contents and method of the course
The proteome of a complex sample shall be analyzed. Therefore, first, the complexity of the sample will be reduced using appropriate separation techniques. After that, an analysis of the sample, applyig mass spectrometry, will be performed. In the course of this practical work, different methods for protein analysis will be presented and practically applied.
Assessment and permitted materials
Performance is monitored during the practical course (interest, cooperation), and furthermore determined on the basis of the quality of the delivered protocol and the acquired knowledge (determined in the closing meeting).
Minimum requirements and assessment criteria
Students need positive grades for each part of the performance (interest/cooperation, protocol and closing meeting regarding the acquired knowledge) in order to successfully complete the course.
Examination topics
Background knowledge on the conducted experiments (provided in two theoretical parts and during practicle course): preparation of biological sample material, isolation of different blood constitutents or different cell types, subcellular fractionation (separation of cytoplasmic and nuclear proteins), protein separation techniques using 2D- and 1D-gel electrophoresis, digestion of proteins into petides, identification of proteins using LC-MS/MS analyses.
Reading list
Additional literature is not required.
Association in the course directory
AN-2, AN-1, BC-1, CHE II-1, LMC-C1, LMC C1
Last modified: Th 03.02.2022 15:49