270119 UE Trennmethoden und MS (2006W)
Hochleistungstrennmethoden und Massenspektrometrie (Wahlfach-PR)
Continuous assessment of course work
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Inst. f. Analyt. Chemie, Abt. Trennmethoden, Vorbesprechung: 03.10.2006, 10.00 Uhr, HS III d. Chem. Institute
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Language: German
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Aims, contents and method of the course
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The hyphenation of high-performance separation techniques (high-performance liquid chromatography, capillary electrophoresis) with mass spectrometry has become one the most important analytical methods for the identification, structure elucidation and quantification of a broad spectrum of analytes (e.g. drugs, biomolecules as e.g. proteins, peptides, metabolites, etc.). During this course current fields of application for HPLC-MS will be demonstrated via an active involvement in ongoing research projects in the group.
Examination topics
Reading list
Association in the course directory
F210
Last modified: Fr 31.08.2018 08:55
· Use of chemoaffinity techniques for the analysis of complex samples of biological origin (e.g. protein mixtures)
· Sample preparation steps, e.g. solid-phase extraction, enzymatic digestion of proteins, chemical modification
· HPLC-MS measurements and computer-assisted data analysis and interpretation