270171 PR Lipidomics practical course (2020W)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Tu 01.09.2020 08:00 to Su 31.01.2021 23:59
- Deregistration possible until Su 31.01.2021 23:59
Details
max. 12 participants
Language: German
Lecturers
Classes
14th to 18th of December, protocols 4 week later and examination 6 weeks later (mid January)
Information
Aims, contents and method of the course
This course will introduce you to the basic concept of lipidomics and will contain lectures but also a lot of practical lipidomics work ranging from sample extraction over the analysis of different lipid classes to their identification and visualization within a given lipidome. More explicitly you will learn about the do and don’ts of lipid sample extraction, familiarize you to the concepts of bottom-up and top-down shotgun lipidomics, analyze your own samples with direct infusion experiments, analyze your data with the latest software and compare your results across different experiments.
Assessment and permitted materials
We will judge your course work and commitment and questionnaire your work in oral examination.
Minimum requirements and assessment criteria
Basic knowledge in data interpretation and statistics, concepts of mass spectrometry (lecture on mass spectrometry)
Examination topics
practical exertion of experiments and teaching material
Reading list
Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics (doi: 10.1194/jlr.D700041-JLR200 )
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
LipidXplorer: a software for consensual cross-platform lipidomics. (doi: 10.1371/journal.pone.0029851)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
LipidXplorer: a software for consensual cross-platform lipidomics. (doi: 10.1371/journal.pone.0029851)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)
Association in the course directory
AN-2
Last modified: We 02.12.2020 09:08