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270175 UE Biochemistry Laboratory Course for Chemists (2019W)

Basic Techniques

5.00 ECTS (5.00 SWS), SPL 27 - Chemie
Continuous assessment of course work

Summary

1 Tiroch, Moodle
2 Martens, Moodle
3 Bachmair, Moodle
4 Hamilton, Moodle

Registration/Deregistration

Registration information is available for each group.

Groups

Group 1

max. 16 participants
Language: German
LMS: Moodle

Registration/Deregistration

  • Registration is open from Th 05.09.2019 08:00 to Th 19.09.2019 18:00
  • Deregistration possible until Th 19.09.2019 18:00

Lecturers

Classes

Verpflichtende Vorbesprechung: 2.10.2019, 10.45 Uhr, SR 1/BZB, Dr.Bohrgasse 9, 1030 Wien.
Termin: 21.-30.10.2019 ganztägig
UE nur für Bachelor Chemie

Aims, contents and method of the course

5 hours (5 credits); laboratory course; F161

Contents: Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

Minimum requirements and assessment criteria

Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

Group 2

max. 16 participants
Language: German
LMS: Moodle

Registration/Deregistration

    Lecturers

    Classes

    Verpflichtende Vorbesprechung: 2.10.2019, 10.45 Uhr, SR 1/BZB, Dr.Bohrgasse 9, 1030 Wien.
    Termin: 20.-29.11.2019 ganztägig
    UE nur für Bachelor Chemie

    Aims, contents and method of the course

    5 hours (5 credits); laboratory course; F161

    Contents: Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation, isolation and restriction-analysis of plasmid DNA.

    Minimum requirements and assessment criteria

    Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

    Group 3

    max. 16 participants
    Language: German
    LMS: Moodle

    Registration/Deregistration

    • Registration is open from Th 05.09.2019 08:00 to Th 19.09.2019 18:00
    • Deregistration possible until Th 19.09.2019 18:00

    Lecturers

    Classes

    Verpflichtende Vorbesprechung: 2.10.2019, 10.45 Uhr, SR 1/BZB, Dr.Bohrgasse 9, 1030 Wien.
    Termin: 07.-17.01.2020 ganztägig
    UE nur für Bachelor Chemie

    Aims, contents and method of the course

    5 hours (5 credits); laboratory course; F161

    Contents: Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

    Minimum requirements and assessment criteria

    Understanding of general principles for planning and carrying out biochemical experiments in the lab.

    Group 4

    max. 16 participants
    Language: German
    LMS: Moodle

    Registration/Deregistration

    • Registration is open from Th 05.09.2019 08:00 to Th 19.09.2019 18:00
    • Deregistration possible until Th 19.09.2019 18:00

    Lecturers

    Classes

    Verpflichtende Vorbesprechung: 2.10.2019, 10.45 Uhr, SR 1/BZB, Dr.Bohrgasse 9, 1030 Wien.
    Termin: 05.-14.02.2019 ganztägig
    UE nur für Bachelor Chemie

    Aims, contents and method of the course

    5 hours (5 credits); laboratory course; F161

    Contents: Lab course featuring cell disruption, fractionated precipitation, protein assays, de-salting, gel filtration, ion exchange and affinity chromatography, radioactive tracer methods, enzyme assays, enzyme kinetics including inhibitor studies, SDS-gel electrophoresis, Western blotting, fractionation of membranes by density gradient centrifugation.

    Minimum requirements and assessment criteria

    Vermittlung der grundlegenden Methoden zur Planung und Durchführung biochemischer Experimente.

    Information

    Assessment and permitted materials

    Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

    Examination topics

    Theoretical background and practical aspects of all experiments performed in the course as well as general biochemical methods.

    Reading list

    Script and additional information as provided on the Moodle plattform.
    Moreover biochemical textbooks such as Stryer.

    Association in the course directory

    BA CH 17b

    Last modified: Th 19.12.2019 12:48