270178 VO Mass Spectrometry Fundamentals and Applications (2011W)
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Cours from 10.01.2012-20.01.2012. Location: TBA.
Beginn: Tuesday 10.01.2012, 10:00, Seminarroom Organics Chemistry.
Beginn: Tuesday 10.01.2012, 10:00, Seminarroom Organics Chemistry.
Details
Language: English
Examination dates
Lecturers
Classes
Currently no class schedule is known.
Information
Aims, contents and method of the course
Assessment and permitted materials
Minimum requirements and assessment criteria
Course objectives:- To understand/revisit the basic principles of mass spectrometry
- To understand the working principles of the main mass spectrometers, including Orbitrap FTMS
- To understand the main MS-based approaches in the analysis of small molecules, either isolated or present in complex mixtures
- To understand the main MS-based approaches in the analysis of large molecules, including peptides and proteins, either isolated or present in complex mixtures
- To get the hands-on experience in peptide sequencing
- To discuss the current MS-based applications in (bio)chemistry and life sciences
- To understand the working principles of the main mass spectrometers, including Orbitrap FTMS
- To understand the main MS-based approaches in the analysis of small molecules, either isolated or present in complex mixtures
- To understand the main MS-based approaches in the analysis of large molecules, including peptides and proteins, either isolated or present in complex mixtures
- To get the hands-on experience in peptide sequencing
- To discuss the current MS-based applications in (bio)chemistry and life sciences
Examination topics
1. Isotopic distributions calculations (classroom)
2. Peptide sequencing calculations (classroom)
Reading list
Association in the course directory
EF-1, EF-2, EF-3, FEG III.
Last modified: We 19.08.2020 08:06
1. Mass spectrometry: a timeline and brief overview of applications
2. Accurate mass and isotopic distribution (+ practical work)
3. MS analytical characteristics: resolution and mass accuracy
4. MS main steps: ionization, ion separation, ion detection
5. Ion sources: electrospray (ESI), MALDI, and others
6. Mass analyzers: 2D and 3D ion traps, time-of-flight MS
7. FT-based mass analyzers: Orbitrap and ICR FTMS
8. Coupling of separation techniques to MS: liquid and gas chromatography
9. Tandem mass spectrometry (MS/MS): methods and techniques
10. Fundamentals of peptide sequencing (+ practical work)
11. MS-based small molecule analysis: metabolomics, petroleomics
12. MS-based protein identification & characterization: proteomics
13. MS-based protein quantification
14. Top-down proteomics, including protein-drug characterization
15. Protein structure analysis: hydrogen-deuterium exchange
16. Protein structure analysis: ion mobility mass spectrometry
17. MS-based imaging