Universität Wien

270194 VO Immunoanalytical Methods (2022W)

2.00 ECTS (1.00 SWS), SPL 27 - Chemie
ON-SITE

Do. 12:00-12:45 im Hörsaal 4, außer Donnerstag, 19.01.2023 von 12:00-12:45 im HS 3

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details


Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;Immunoprecipitation; Immunohistochemistry;Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays. Data processing: construction of the calibration curve, quality control.Lateral flow devicesImmuno-PCR

Assessment and permitted materials

oral exam, individual appointment

Minimum requirements and assessment criteria

Passing threshold at 50%50-62% "genügend", 62,5-75% "befriedigend", 75,5-88% "gut", darüber "sehr gut"

Examination topics

PowerPoint slides (Moodle)

Reading list

Wild, The Immunoassay Handbook, 2013, Elsevier

Association in the course directory

AN-1, A.5, AN-2, BC-1, CH-SAS-05

Last modified: We 15.11.2023 15:28