270201 PR Targeted Lipidomics (2024S)
Continuous assessment of course work
Labels
ON-SITE
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Sa 03.02.2024 08:00 to Mo 26.02.2024 23:59
- Deregistration possible until Mo 26.02.2024 23:59
Details
max. 10 participants
Language: German, English
Lecturers
Classes
11th to 13th of March introduction online and laboratory work (Part I)
18th to 20th of March (Part II)
or 8th to 10 of April (Part II)
Information
Aims, contents and method of the course
Lipidomics is the science that deals with the concentration-dependent description of hydrophobic biomolecules in space and time in a specific system. You will be taught one of the most practical approaches to achieve this goal. This course focuses on LC-MS based lipidomics. However, this course will also deliver basic concepts how to identify small molecules, which you can also apply in other fields of analytical chemistry. In this course the following topics might be presented or worked out together: lipid structure and function, nomenclature of lipids, extraction and sample preparation, LC-MS lipidomics, evaluation strategies for identification and quantification. Critical examination and scientific discussion of research results.
Assessment and permitted materials
We will judge your coursework and commitment. At the end of the course, you must present your lab results in a short presentation.
Minimum requirements and assessment criteria
Basic knowledge in data interpretation and statistics, concepts of mass spectrometry (lecture on mass spectrometry).
Examination topics
Coursework
Commitment
Presentation of lab results
Commitment
Presentation of lab results
Reading list
Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics (doi: 10.1194/jlr.D700041-JLR200 )
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)
Top-down lipidomic screens by multivariate analysis of high-resolution survey mass spectra.( doi: 10.1021/ac062455y)
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.( doi: 10.1021/ac102505f)
Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome.( doi: 10.1182/blood-2017-12-822890.)
Association in the course directory
CH-SAS-06,BC CHE II-1, Design
Last modified: Fr 08.03.2024 09:46