270281 VO Immunanalytische Methoden (2006S)
Immunanalytische Methoden
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Mo. 12:15-13:00 HS III d. Chem. Inst., Beginn. 06.03.2006
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Introduction into basics of immunoanalytical methods
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F213,LA-Ch234
Last modified: Fr 31.08.2018 08:55
Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Typical applications: pregnancy, BSE, HIV.