270281 VO Immunoanalytical Methods (2010W)

1.00 ECTS (1.00 SWS), SPL 27 - Chemie

Do 10:00-11:00 Uhr c.t., HS IV d. Chem. Inst., Beginn: 07.10.2010.

Details

Language: German

Examination dates

Lecturers

Margit Cichna-Markl

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Immuno-PCR

Assessment and permitted materials

Minimum requirements and assessment criteria

Introduction into basics of immunoanalytical methods

Examination topics

Evaluation: written examination

Reading list

Association in the course directory

AN-1, CHE II-1, BC-1, LA-Ch.

Last modified: We 19.08.2020 08:06