270281 VO Immunoanalytical Methods (2015W)

1.50 ECTS (1.00 SWS), SPL 27 - Chemie

Moodle

Details

max. 50 participants

Language: German

Examination dates

Lecturers

Margit Cichna-Markl

Classes

Do, 10:15 bis 11:00

Kleiner Hörsaal 4
1090, Währinger Straße 42, Halbstock

Beginn: 08.10.2015

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Immuno-PCR

Assessment and permitted materials

Minimum requirements and assessment criteria

Introduction into basics of immunoanalytical methods

Examination topics

Evaluation: written examination

Reading list

Association in the course directory

AN-1, AN-4, 2LA-Ch 32-34, A.5;

Last modified: Mo 07.09.2020 15:42