Universität Wien

270281 VO Immunoanalytical Methods (2018W)

1.50 ECTS (1.00 SWS), SPL 27 - Chemie
Moodle

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Details

max. 50 participants
Language: German

Examination dates

Lecturers

Classes (iCal) - next class is marked with N

  • Thursday 04.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 11.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 18.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 25.10. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 08.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 15.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 22.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 29.11. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 06.12. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 13.12. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 10.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 17.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 24.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42
  • Thursday 31.01. 10:00 - 11:00 Hörsaal 4 Chemie HP Währinger Straße 42

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Immunoprecipitation; Immunohistochemistry;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Lateral flow devices
Immuno-PCR

Assessment and permitted materials

Written Exam

Minimum requirements and assessment criteria

Basics of immunoanalytical methods

Examination topics

Evaluation: written examination

Reading list

Wild, The Immunoassay Handbook, 2013, Elsevier

Association in the course directory

AN-1, AN-4, 2LA-Ch 32-34, A.5

Last modified: Mo 07.09.2020 15:42