270281 VO Immunoanalytical Methods (2020W)

Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

Language: German

Examination dates

Lecturers

Margit Cichna-Markl

Classes

online (BigBlueButton)
every Thursday, 10:15-11:00

Information

Aims, contents and method of the course

Basic immunology: Immune response, structure of antibody molecules, antibody-antigen interactions, production and purification of antibodies (polyclonal, monoclonal, recombinant), characterisation of antibodies (affinity, specificity, cross reactivity), immunoaffinity chromatography;
Immunoprecipitation; Immunohistochemistry;
Principle of immunoassays and immunometric assays: labelling of antigens or antibodies, radioimmunoassay (tritium, I-125), enzyme immunoassay, fluoro-immunoassay, DELFIA, development and optimisation of immunoassays.
Data processing: construction of the calibration curve, quality control.
Lateral flow devices
Immuno-PCR

Assessment and permitted materials

oral digital exam, individual appointment

Minimum requirements and assessment criteria

Passing threshold at 50%
50-62% "genügend", 62,5-75% "befriedigend", 75,5-88% "gut", darüber "sehr gut"

Examination topics

PowerPoint slides (Moodle)

Reading list

Wild, The Immunoassay Handbook, 2013, Elsevier

Association in the course directory

AN-1, 2LA-Ch 32-34, A.5

Last modified: Th 28.10.2021 16:49