270297 VO Receptor-Ligand Interactions and Determination of Association Constants (2010S)

1.00 ECTS (1.00 SWS), SPL 27 - Chemie

Seminarraum d. Inst. f. Analyt. Chemie, Termin wird bei Vorbesprechung: Di, 02.03.2010, 10.15 Uhr, HS III d. Chem. Institute festgelegt.

Details

Language: German

Examination dates

Lecturers

Michael Lämmerhofer

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

Prinziples of protein-ligand interactions (bindung contributions, thermodynamics and binding energetics), principal experimental protocols for the determination of bindung constants (steady-state measurements, kinetic measurements, competition experiments), linear transformations (Scatchard plot), stoichiometry, allosteric effects, cooperativity;
methods for the determination of binding constants: microcalorimetry, radioreceptor assay, chromatographic methods (including static (batch) method for the determination of adsorption isotherms, frontal analysis, Hummel-Dreyer Method), elektrophoretic methods (affinity CE), spectroscopic methods (NMR, UV, fluorescence, CD), surface-plasmon resonance

Assessment and permitted materials

Minimum requirements and assessment criteria

The goal is that the students get knowledge of the various possibilities for the determination of binding constants by distinct techniques. The course should teach students about the general principles, technical aspects, potential sources for errors and the applicability range of the different techniques.

Examination topics

Evaluation: colloquium

Reading list

Association in the course directory

AN-1, CHEII-1, BC-1, LA-Ch 232-234.

Last modified: We 19.08.2020 08:06