Universität Wien FIND

300010 UE Advanced techniques of electronmicroscopy and ultrastructural research (2020S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 8 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

The preliminary Meeting: Tuesday, March 3, 2020 at 1 p.m. in the Conference Room of Zoology. The Course itself starts on Monday, March 23, 2020 at 8:15 a.m. in the Conference Room of Zoology with the introductory lecture. Work in the EM-Lab starts then at 9:15 a.m. in the Core Facility Cell Imaging and Ultrastructure Research, Floor 1, EM-Lab. The practical Course lasts for 2 weeks en bloc, starting from March 23, 2020 to April 3, 2020. Daily lectures from 8:15 -9 o'clock, daily practicals in the lab from 9:15 a.m. 4:00 p.m. After the second week final work can be done at the Instruments, at the Computer, at the Protocols and the final Reports. During this time presence is not compulsory, but attendance is requested during the first weeks of intensive training at the electron microscopes and adjacent techniques.

Friday 14.08. 09:00 - 10:30 Seminarraum 3
Monday 17.08. 08:15 - 09:00 Seminarraum 3
Tuesday 18.08. 08:15 - 09:00 Seminarraum 3
Wednesday 19.08. 08:15 - 09:00 Seminarraum 3
Thursday 20.08. 08:15 - 09:00 Seminarraum 3
Friday 21.08. 08:15 - 09:00 Seminarraum 3
Monday 24.08. 08:15 - 09:00 Seminarraum 3
Tuesday 25.08. 08:15 - 09:00 Seminarraum 3
Wednesday 26.08. 08:15 - 09:00 Seminarraum 3
Thursday 27.08. 08:15 - 09:00 Seminarraum 3
Friday 28.08. 08:15 - 09:00 Seminarraum 3

Information

Aims, contents and method of the course

The main topic of this electron microscopy course is the detection of heavy metals and other chemical elements in plant- and animal- cells and tissues by means of energy dispersive X-ray microanalysis (EDX). An introduction is also given in electron energy loss spectroscopy (EELS), the use of unstained ultrathin, and of semithin sections in the TEM and SEM. The investigations are made on the filtering TEM Zeiss EM 902 and TEM Zeiss Libra 120, as well as on the digital SEM Philips XL-30 and JEOL JSM IT300. Preparation methods freeze-drying, carbon-coating, ultrathin sectioning by ultramicrotome and contrasting of sections on grids for TEM.

Assessment and permitted materials

Presentation of a project on heavy metal contamination and stress of animals and plants. An additional aim is the knowledge of special SEM and TEM-methods and of techniques for sample preparation.

Minimum requirements and assessment criteria

Lecture 25%, Lab- and Microscopy work 25%, Group-Protocol 25%, Oral report at final seminar 25%

Examination topics

The results of the experiments will be discussed and the advanced techniques of electron microscopy will be dealt with , such as EELS, ESI, Contrast Tuning, ESEM, EDX, BSE, Dot-Mapping, semithin and ultrathin sectioning on the Ultramicrotome, advanced SEM and TEM. The students' understanding of all this will be judged by the group-protocol and the oral report in the final seminar.

Reading list

Will be available during the course

Association in the course directory

WZB, UF MA BU 04, MZO 2, MEC-9 , MBO 7

Last modified: Sa 22.10.2022 00:29