Universität Wien FIND

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300010 UE Advanced techniques of electronmicroscopy and ultrastructural research (2021S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
ON-SITE

Registration/Deregistration

Details

max. 8 participants
Language: German

Lecturers

Classes (iCal) - next class is marked with N

The preliminary Meeting: Tuesday, March 2, 2021 at 1:15 p.m. probably online, see moodle please! The Course itself starts on Monday, March15, 2021 at 8:15 a.m. with the introductory lecture online. Work in the EM-Lab starts then at 10 a.m. in the Core Facility Cell Imaging and Ultrastructure Research, Floor 1, EM-Lab. The practical Course lasts for 2 weeks en bloc, starting from March 15, 2021 to March 26, 2021. Daily lectures from 8:15 -9 o'clock, daily practicals in the lab from 10:00 a.m. 4:00 p.m. After the second week final work can be done at the Instruments, at the Computer, at the Protocols and the final Reports. During this time presence is not compulsory, but attendance is requested during the first weeks of intensive training at the electron microscopes and adjacent techniques, probably 1 person after the other alone with 1 teacher for 1 hour each!

Friday 26.03. 10:00 - 12:00 Seminarraum 3, UZA 1, Biozentrum Althanstraße 14, Z2.027 1.OG

Information

Aims, contents and method of the course

The main topic of this electron microscopy course is the detection of heavy metals and other chemical elements in plant- and animal- cells and tissues by means of energy dispersive X-ray microanalysis (EDX). An introduction is also given in electron energy loss spectroscopy (EELS), the use of unstained ultrathin, and of semithin sections in the TEM and SEM. The investigations are made on the filtering TEM Zeiss EM 902 and TEM Zeiss Libra 120, as well as on the digital SEM Philips XL-30 and JEOL JSM IT300. Preparation methods freeze-drying, carbon-coating, ultrathin sectioning by ultramicrotome and contrasting of sections on grids for TEM.

Assessment and permitted materials

Presentation of a project on heavy metal contamination and stress of animals and plants. An additional aim is the knowledge of special SEM and TEM-methods and of techniques for sample preparation.

Minimum requirements and assessment criteria

Lecture 25%, Lab- and Microscopy work 25%, Group-Protocol 25%, Oral report at final seminar 25%

Examination topics

The results of the experiments will be discussed and the advanced techniques of electron microscopy will be dealt with , such as EELS, ESI, Contrast Tuning, ESEM, EDX, BSE, Dot-Mapping, semithin and ultrathin sectioning on the Ultramicrotome, advanced SEM and TEM. The students' understanding of all this will be judged by the group-protocol and the oral report in the final seminar.

Reading list

Will be available during the course

Association in the course directory

WZB, UF MA BU 04, MZO 2, MEC-9 , MBO 7

Last modified: We 05.05.2021 09:48