Universität Wien FIND

300052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2016S)

for diploma, PhD and advanced students

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann, Moodle
2 Gotzmann, Moodle

Registration/Deregistration

Groups

Group 1

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

Preliminary Meeting (attendance mandatory): Wed, March 9th, 10-10.30am; BZB/SR 3-6th floor, Dr.Bohrgasse 9.1030 Wien.

Limited Attendance: 8 people per course (in total 16)

Course dates (presence at all days mandatory): April: 1.,13.,14.,20.,21.,26.,27.,28; May 3.+4; 2016
Location: MFPL-VBC5, Dr. Bohrg. 9, 1030 Vienna

Registration for one of the two offered Course Groups is SUFFICIENT.
Simple UNIVIS registration is NOT necessarily a ticket for the course. Participants will be selected based on the questionnaire (see below) results by the course manager and will be announced at the preliminary meeting.
Students with experience in light microscopy AND those who have a proven demand for their master/PhD-work will be preferentially selected.
Please fill in questionnaire and send it to josef.gotzmann@univie.ac.at
download questionnaire at: http://molekularebiologie.univie.ac.at/fileadmin/user_upload/zentrum_molekulare_biologie/Formulare/questionaire_english-confocal_course-Gotzmann-300052-SS2016.doc
Details for program and schedule at preliminary meeting.

Assessment and permitted materials

oral exam and continuous assessment

Group 2

max. 8 participants
Language: German
LMS: Moodle

Lecturers

Classes

Preliminary Meeting (attendance mandatory): Wed, March 9th, 10-10.30am; BZB/SR 3-6th floor, Dr.Bohrgasse 9.1030 Wien.

Limited Attendance: 8 people per course (in total 16)

Course dates (presence at all days mandatory): April: 1.,13.,14.,20.,21.,26.,27.,28; May 3.+4; 2016
Location: MFPL-VBC5, Dr. Bohrg. 9, 1030 Vienna

Registration for one of the two offered Course Groups is SUFFICIENT.
Simple UNIVIS registration is NOT necessarily a ticket for the course. Participants will be selected based on the questionnaire (see below) results by the course manager and will be announced at the preliminary meeting.
Students with experience in light microscopy AND those who have a proven demand for their master/PhD-work will be preferentially selected.
Please fill in questionnaire and send it to josef.gotzmann@univie.ac.at
download questionnaire at:http://molekularebiologie.univie.ac.at/fileadmin/user_upload/zentrum_molekulare_biologie/Formulare/questionaire_english-confocal_course-Gotzmann-300052-SS2016.doc
Details for programme and schedule at preliminary meeting.

Assessment and permitted materials

oral exam and continuous assessmen

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy

technical description of equipment

Wet lab experience sample preparation
Quality criteria for dealingwith aberrations correction

practical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)

image analysis and deconvolution of selected images

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

PhD-MB 2, MMEI III, MMB W-2, WZB

Last modified: Fr 31.08.2018 08:56