Universität Wien FIND
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300052 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2017S)

for diploma, PhD and advanced students

3.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Summary

1 Gotzmann , Moodle
2 Gotzmann , Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
Registration information is available for each group.

Groups

Group 1

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Preliminary Meeting: Thu, March 2nd, 11.00am-11.30am (attendance mandatory)
Sem room 3, 6th floor, MFPL

Registration for one of the two courses offered sufficient. Simple registration does not necessarilly mean a fixed seat.
In case of overbooking the head of the course will selcet people on the basis of career status and the demand on performing microscopic experiments in the course of a Master/PhD-thesis in a group.
Seat limitation: 8 (per course; in total 16)
More schedule and programme details at the preliminary meeting.

Dates: all days are full-day (unless otherwise noted)
Course1: March, 14,15,16,21,23; April 4,5 (Mandatory attendance at all days)
Course 2: March 14+15 (9-12.30 only); March 16,28,29,30; April 4+5 (Mandatory attendance at all days)

Location: VBC5, MFPL, Dr. Bohrg.9, 1030 Vienna

Assessment and permitted materials

oral exam and continuous assessment

Group 2

In case non-German speaking students participate we, of course, will teach in English!

max. 8 participants
Language: German, English
LMS: Moodle

Lecturers

Classes

Preliminary Meeting: Thu, March 2nd, 11.00am-11.30am (attendance mandatory)
Sem room 3, 6th floor, MFPL

Registration for one of the two courses offered sufficient. Simple registration does not necessarilly mean a fixed seat.
In case of overbooking the head of the course will selcet people on the basis of career status and the demand on performing microscopic experiments in the course of a Master/PhD-thesis in a group.
Seat limitation: 8 (per course; in total 16)
More schedule and programme details at the preliminary meeting.

Dates: all days are full-day (unless otherwise noted)
Course1: March, 14,15,16,21,23; April 4,5 (Mandatory attendance at all days)
Course 2: March 14+15 (9-12.30 only); March 16,28,29,30; April 4+5 (Mandatory attendance at all days)

Location: VBC5, MFPL, Dr. Bohrg.9, 1030 Vienna

Assessment and permitted materials

oral exam and continuous assessmen

Information

Aims, contents and method of the course

Theoretical basis of fluorescence and confocal microscopy

technical description of equipment

Wet lab experience sample preparation
Quality criteria for dealingwith aberrations correction

practical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)

image analysis and deconvolution of selected images

Minimum requirements and assessment criteria

The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.

Examination topics

microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software

Reading list

J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008

Association in the course directory

PhD-MB 2, MMEI III, MMB W-2, WZB

Last modified: Mo 07.09.2020 15:43