300091 UE Course III A - Laboratory Work in Molecular Biology (2009W)

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Maximal number of participants: 20

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Maximal number of participants: 20

Special examples of experiments in molecular biology, practical implementation of methods reviewed in the seminar: cloning of genes (including PCR amplification) and expression in E. coli, detection of the protein by western blot analysis. Isolation of a gene, labelling and use in Southern blot analysis.
Maximal number of participants: 20

This course is designed to trained students in state-of-the-art techniques in RNA biochemistry. They will work on small projects: i) Protein-facilitated RNA folding in vivo; ii) The effect of ionic strength and temperature on RNA structure; iii) Determining the affinity of RNA-protein interactions. The methods involved include RNA isolation from cells, primer extension, in vitro transcription, ribozyme cleavage assays, and native gel-electrophoreses (EMSA). The course is accompanied by a seminar, which is designed to convey fundamental principles of RNA structure, folding and interaction with ligands such as metal ions and proteins. In addition, the content of teaching will demonstrate the diversity of biochemical and biophysical RNA methodologies.