Universität Wien

300094 UE Proteomics in Systems Biology (2023S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

Preliminary meeting will be online, March 1st, 10am (zoom link will be send day before). If you cannot join, please notify Prof. Wienkoop and try to get informations otherwise. NOTE: If you are not present and do not notify Prof. Wienkoop, the place will automatically given to the next on the waiting list!
An introductory lecture by Prof. Weckwerth March 2, 11am via zoom (~2h)
The course will be from March 6th to 17th.

  • Monday 06.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Tuesday 07.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 08.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 09.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 10.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Tuesday 14.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Wednesday 15.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Thursday 16.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
  • Friday 17.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1

Information

Aims, contents and method of the course

The terms "proteome" and "metabolome" (entirety of all active proteins and metabolites in a cell or tissue) was coined 10 years ago in analogy to the "genome" (entirety of all genes). The proteome comprises the full suite of all proteins/enzymes in a cell or a tissue. Because of the diversity and dynamic of the proteome sophisticated bioanalytical methods, especially mass spectrometry, are needed. In the frame of the project the student should learn basics of the technology proteomics and a special mass spectrometric technique for the analysis of proteins (shotgun proteomics for identification and quantification). At the same time the course will impart knowledge about mass spectrometrie in modern biology.

Assessment and permitted materials

Collaboration (exercise [questionnaire], discussion) 30%
Labwork 20%
Protocol and final presentation (25% each)

Minimum requirements and assessment criteria

english

Examination topics

Protein extraction,- digestion, -separation (HPLC and SDS-PAGE), Mass Spectrometry for protein ID and quantification, data mining

Reading list

Association in the course directory

BBO 9, BOE 12, MMB W-2, MMEI III, MBO 7, MEC-9, M-WZB, MES5

Last modified: Tu 14.03.2023 12:09