300094 UE Proteomics in Systems Biology (2025S)
Continuous assessment of course work
Labels
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, muss diese absolviert werden. Der link zum entsprechenden Moodle-Kurs wird ihnen nach Aufnahme in den Kurs zugesendet.
Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safety instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
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Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, falls benötigt, muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safety instruction for this course. After the registration period, the admitted students have to complete this instruction. The link to the corresponding Moodle course will be sent to you after you have been accepted to the course.
All practice rooms may only be entered with a lab coat and closed shoes, and, if needed, lab glasses must also be purchased - please store private belongings in the cloakroom.
_
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 06.02.2025 14:00 to Th 20.02.2025 18:00
- Deregistration possible until Sa 15.03.2025 18:00
Details
max. 16 participants
Language: English
Lecturers
Classes (iCal) - next class is marked with N
The lab course will be from March 17. - 28. Independent self-learning the week before!
Preliminary meeting via zoom, March 3rd at 2 pm. A link will be send out the day before. It is mandatory to attend (!) - notify Prof. Wienkoop if you cannot make it. ideally send a colleague in case you are prevented. Otherwise the space might be given to the next on the waiting list.
Students on waiting list may also attend as there might be spare space.
- Monday 17.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
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Tuesday
18.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Wednesday
19.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Thursday
20.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Friday
21.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 - Monday 24.03. 09:00 - 17:00 Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
-
Tuesday
25.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Wednesday
26.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Thursday
27.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1 -
Friday
28.03.
09:00 - 17:00
Übungsraum 1.7 Physiologie 2, Biologie Djerassiplatz 1, 1.041, Ebene 1
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Information
Aims, contents and method of the course
The terms "proteome" and "metabolome" (entirety of all active proteins and metabolites in a cell or tissue) was coined 10 years ago in analogy to the "genome" (entirety of all genes). The proteome comprises the full suite of all proteins/enzymes in a cell or a tissue. Because of the diversity and dynamic of the proteome sophisticated bioanalytical methods, especially mass spectrometry, are needed. In the frame of the project the student will learn protein extraction and basics of the technology proteomics and a special mass spectrometric technique for the analysis of proteins (shotgun proteomics for identification and quantification). At the same time the course will impart knowledge about mass spectrometrie in modern biology.
Assessment and permitted materials
Collaboration (exercise [questionnaire] 10%, discussion 15%) 25%
Labwork 25%
Protocol and final presentation (in groups) (25% each)
Labwork 25%
Protocol and final presentation (in groups) (25% each)
Minimum requirements and assessment criteria
english
Examination topics
Protein extraction,- digestion, -separation (HPLC and SDS-PAGE), Mass Spectrometry for protein ID and quantification, data mining and presentation
Reading list
Literature will be uploaded to moodel!
Association in the course directory
MBO 7, MEC-9, MES5, MMEI III
Last modified: Th 30.01.2025 11:06