Universität Wien

300113 UE Transmission Electron Microscopy of Cells and Tissues -Techniques and Preparation (2023S)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: German, English

Lecturers

Classes (iCal) - next class is marked with N

Mo. 13.03.2023: 10:00-11:00 Short introduction to course modalities via Zoom-meeting. Participants will receive a Zoom-Link in time.

Mo 20.03.2023 Seminar room 1.3 UBB
09:00 – 10:00
Powerpoint talk: The way of samples in the transmission electron microscope
11:00- 12:00
Powerpoint talk: Explanation of the practical task – TEM preparation of nauplii
13:00- 14:00
Powerpoint talk: Chemical fixation and dehydration
15:00-16:00
Powerpoint talk: Embedding in resin

Tu. 21.03.2023
Praktical work in the EM-Lab at the CIUS
09:00- 09:30: Safety tour with Daniela Gruber
09:30- 16:30: Preparation of glass knives, Formvar coating of grids, introduction to microwave- and cryofixation, and freeze substitution

We. 22.03.2023
Demos in preparation of the practical work
09:00- 10:00: Demo- Trimming of samples
10:30- 11:30: Demo- Semithin sectioning with an ultramicrotome for light microscopy
12:30- 13:30: Demo - Ultrathin sectioning with an ultramicrotome for TEM
14:00- 15:00: Demo- contrasting of sections
15:30- 16:00: Demo- work with the transmission electron microscope

Th. 23.03.2023
Practical work in the EM-Lab (CIUS)
9:30- 16:30: Sectioning with an ultramicrotome, contrasting, introduction to the TEM.

Fr. 24.03.2023
Practical work in the EM-Lab (CIUS)
9:30-16:30: Sectioning with an ultramicrotome, contrasting, TEM.

Mo. 27.03.2023
Praktical work in the EM-Lab (CIUS)
09:30-16:30: Sectioning with an ultramicrotome, contrasting, TEM

Tu. 28.03.2023
Practical work in the EM-Lab (CIUS)
9:30- 16:30: Contrasting, light microscopy, TEM

Fr. 31.03.2023
Finale
09:30-14:00: Computer room 4.076 talks, final discussion

If Corona- measures are required, powerpoint talks, demos and the final discussion will be held via video conferences.

Monday 20.03. 08:00 - 16:30 Seminarraum 1.3, Biologie Djerassiplatz 1, 1.005, Ebene 1

Information

Aims, contents and method of the course

Transmission Electron Microscopy (TEM) has been proven as a valuable tool for a variety of research topics in life sciences. This course provides skills in sample preparation and the use of TEMs. It should enable students to work independently with their own samples.The following preparation steps will be teached: Chemical fixation (glutaraldehyde, paraformaldehyde, OsO4), dehydration, embedding in epoxy resin, thin-and ultrathin sectioning with ultramicrotomes, contrasting of sections, evaluation and interpretation of the results in the TEM.A preview will be given concerning advanced sample preparation techniques based on cryotechniques, which all build up on basic skills trained during our course.

Assessment and permitted materials

Every item has to be positive:
Moodle test safety in the EM-lab: 25 points
(Test is precondition for participation)
Participation: 50 points
Oral presentation: 25 points

0 - 50 points grade 5
51 - 67 points grade 4
67 - 81 points grade 3
82 - 90 points grade 2
91 - 100 points grade 1

Minimum requirements and assessment criteria

Theoretical knowledge (lecture). Application of equipment for sample preparation and TEMs (practical work). Evaluation, discussion and interpretation of the results.

Examination topics

Ability to select suitable methods for TEM sample preparation, work in the chemistry lab, handling of equipment for sample preparation incl. ultrathin sectioning with ultramicrotomes, work with TEMs, interpretation and evaluation of the results.

Reading list

M. A. Hayat (2000). Principles and Techniques of Electron Microscopy- Biological Applications. 4th Edition, Cambridge University Press, isbn: 9780521632874

Association in the course directory

M-WZB, MZO 2, MZO4, MEC-9, MBO 7, MZO2

Last modified: Mo 20.03.2023 08:28