Universität Wien

300140 VO+UE Live imaging course (2015S)

for diploma, PhD and advanced students

1.00 ECTS (1.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Preliminary meeting: March 5th, 2015, 12.00-13.00 (sharp); BZB/SR3 - 3rd floor, Dr. Bohrgasse 9; 1030 Vienna. Participation mandatory.
Registration via UNIVIS does NOT necessarily mean you have a seat fixed! People will be selected based on the evaluation of questionnaires by the head of the course. A preference will be given to people with light microscopy experience and a real demand on live imaging for their current/future studies. The final assignment of seats will be announced in the preliminary meeting. Please fill in questionnaires as soon as possible and send them to Josef.gotzmann@univie.ac.at; otherwise you cannot participate.
Download at: .........................
Number of attendants limited to 8 people.
Details to programme and workflow in the preliminary meeting.
Dates for the course: June 2nd & 3rd (full day! Attendance on all days obligatory). Location: MFPL-VBC5, Dr. Bohrg. 9, 1030 Vienna.
More Infos: http://molekularebiologie.univie.ac.at/
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 8 participants
Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

The Live Imaging workshop intends to give students a theoretical and practical input on important considerations of live imaging using modern light microscopic technologies.

- Lecture 1: Live Imaging Considerations
- Lecture 2: Live Imaging Dyes and Fluorophores
hands-on planning of overnight and individual shorter time lapse experiments using widefield, spinning disc and confocal microscope setups

Assessment and permitted materials

Continuous Assessment/Oral exam

Minimum requirements and assessment criteria

Students shall learn the theoretical background of imaging in a live regimen. This includes knowledge on instrumental and technological theory, combined with considerations on practical do's and don'ts. The latter shall finally be applied in practical hands-on-sessions using two different live microscopy setups.

Examination topics

a. Students will understand the theory behind basic and advanced light microscopy technologies
b. Students will get a deeper insight into the applicative potential of diverse light microscopy techniques for live imaging
c. Students will learn to transfer knowledge on microscopy hardware and fluorescent molecule selection into an experimental realization
d. Students will learn to critically review, troubleshoot and/or optimize live imaging experiments
e. Students will have to learn and work in cooperative teams

Reading list

Yuste, Live Imaging; A Laboratory manual; CSHL-Press, 2010
Helmchen et al., Imaging in Neuroscience-A Laboratory Manual; CSHL Press, 2011
Sharpe, Imaging in Developmental Biology – A Laboratory manual; CSHL-Press, 2011
Goldman, Swedlow, Spector: Live Cell Imagign-A Laboratory manual; CSHL Press 2009
http://www.microscopyu.com/
http://www.olympusmicro.com/index.html
http://zeiss-campus.magnet.fsu.edu/index.html
http://www.leica-microsystems.com/science-lab/

Association in the course directory

PhD-MB 2, MMEI III, MMB W-2, WZB

Last modified: Mo 07.09.2020 15:43