Universität Wien

300153 UE Practical Course in plantdevelopment and biotechn. (2007S)

Practical Course in Compulsory Subject plantdevelopment and biotechnology

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Für Studenten der Molekul. Biol.: Schwerpunktsfachübung.
Ort: ÜS 1, BZB, Dr. Bohr-Gasse 9, 1030 Wien.
Block: 3 Wochen ganztägig.
Anmeldungen, Beginn- u. Vorbesprechungstermine siehe auch Studentenseite der Departments Dr. Bohr-Gasse: http://www.students.mfpl.ac.at/

Details

Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

1. Plant morphogenesis and embryogenesis in vitro:- organogenesis in petunia leaf discs and stem segments;
- embryogenesis and haploid plant regeneration from tobacco microspores;
- embryogenesis and haploid plant regeneration from wheat microspores;
- plant protoplast isolation and culture.

2.Techniques of plant transformation:
- Agrobacterium tumefaciens mediated gene transfer into tobacco and petunia;
- Direct gene transfer into tobacco protoplasts;
- Floral dip transformation of Arabidopsis thaliana;
- Particle bombardment mediated gene transfer into tobacco microspores;
- Particle bombardment mediated gene transfer into wheat meristems and embryos;
- Transient expression of screenable marker genes:
a) expression of the GUS gene in plant cells. Histochemical GUS assay;
b) expression of the GFP gene in plant cells.

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

M208, M203

Last modified: Fr 31.08.2018 08:56