300153 UE Practical Course in Plantdevelopment (2008S)

1. Plant morphogenesis and embryogenesis in vitro:- organogenesis in petunia leaf discs and stem segments;
- embryogenesis and haploid plant regeneration from tobacco microspores;
- embryogenesis and haploid plant regeneration from wheat microspores;
- plant protoplast isolation and culture.

2.Techniques of plant transformation:
- Agrobacterium tumefaciens mediated gene transfer into tobacco and petunia;
- Direct gene transfer into tobacco protoplasts;
- Floral dip transformation of Arabidopsis thaliana;
- Particle bombardment mediated gene transfer into tobacco microspores;
- Particle bombardment mediated gene transfer into wheat meristems and embryos;
- Transient expression of screenable marker genes:
a) expression of the GUS gene in plant cells. Histochemical GUS assay;
b) expression of the GFP gene in plant cells.