Universität Wien

300153 UE Practical Course in developmental biology of plants (2009S)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Ort: BZB, Dr. Bohr-Gasse 9/8.Stock, 1030 Wien.
Block: 1.-11.9.2009
Contact: AoProf. Alisher Touraev, alisher.touraev@univie.ac.at
Siehe auch Studentenseite des Zentrums für Molekulare Biologie:
http://www.students.mfpl.ac.at/

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 12 participants
Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

I. Media preparation, introduction to tissue culture
II. Plant cell cultures in vitro to study morphogenesis and embryogenesis:

- Direct embryogenesis in isolated and cultured tobacco microspores (dicot model);
- Embryogenesis and plant regeneration in isolated wheat microspores (monocot model);
- Embryogenesis in isolated Brassica microspores
- Microspore cultures of Arabidopsis
- Morphogenesis in cultured tobacco leaf discs: impact of growth regulators on the mode of development

III. Basics of light and fluorescence microscopy to study cultured and
developing plant cell:

- Light and fluorescence microscopy to study in vitro cultured tobacco and
Brassica microspores at different developmental stages.

IV. Staining techniques used in plant cell biology to study cell viability,
nuclear status, cell wall and callose)

- FDA staining of isolated microspores, and pollen to study cell viability;
- DAPI staining of microspores and pollen to study the nucleus status;
- Calcofluor White and Aniline Blue staining of pollen and pollen tube to visualize cell wall and callose.

Assessment and permitted materials

Minimum requirements and assessment criteria

Examination topics

Reading list


Association in the course directory

MPF II-2

Last modified: Mo 07.09.2020 15:43