Universität Wien FIND
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300156 UE Advanced techniques of electronmicroscopy and ultrastructural research (2019S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 8 participants
Language: German

Lecturers

Classes

Preliminary meeting: Tuesday, 13:00 hr (1 p.m.) in the conference room of zoology.
Begin of the course: Monday, March 25, 2019 at 8:15 a.m. in the conference room of zoology with the first lecture. Laboratory work begins at 9:15 a.m.in the Core Facility of Cell Imaging and Ultrastructure Research (EM_Lab), Floor 1, Section 2. The course lasts 3 weeks, from March 25 - April 12, 2019 lectures and practical course will be mainly in the first and second week, starting at 8:15 a.m. with the lecture. The third week will be for evaluation of results, personal work at the instruments, protocol and final report preparation.


Information

Aims, contents and method of the course

The main topic of this electron microscopy course is the detection of heavy metals and other chemical elements in plant- and animal- cells and tissues by means of energy dispersive X-ray microanalysis (EDX). An introduction is also given in electron energy loss spectroscopy (EELS), the use of unstained ultrathin, and of semithin sections in the TEM and SEM. The investigations are made on the filtering TEM Zeiss EM 902 and TEM Zeiss Libra 120, as well as on the digital SEM Philips XL-30 and JEOL JSM IT300. Preparation methods freeze-drying, carbon-coating, ultrathin sectioning by ultramicrotome and contrasting of sections on grids for TEM.

Assessment and permitted materials

Workshop 25%, Lab- and Microscopy work 25%, Protocol 25%, Oral report at final seminar 25%

Minimum requirements and assessment criteria

Presentation of a project on heavy metal contamination and stress of animals and plants. An additional aim is the knowledge of special SEM and TEM-methods and of techniques for sample preparation.

Examination topics

The results of the project- experiments will be discussed and the advanced techniques of electron microscopy will be dealt with , such as EELS, ESI, Contrast Tuning, ESEM, EDX, BSE, Dot-Mapping, semithin and ultrathin sectioning on the Ultramicrotome, advanced SEM and TEM.

Reading list

Will be offered during the course

Association in the course directory

WZB, UF MA BU 04, MZO 2, MEC-9 , MBO 7

Last modified: Th 13.06.2019 14:29