300156 UE Advanced techniques of electronmicroscopy and ultrastructural research (2019S)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Fr 08.02.2019 08:00 to Th 21.02.2019 18:00
- Deregistration possible until Su 31.03.2019 18:00
Details
max. 8 participants
Language: German
Lecturers
Classes
Preliminary meeting: Tuesday, 13:00 hr (1 p.m.) in the conference room of zoology.
Begin of the course: Monday, March 25, 2019 at 8:15 a.m. in the conference room of zoology with the first lecture. Laboratory work begins at 9:15 a.m.in the Core Facility of Cell Imaging and Ultrastructure Research (EM_Lab), Floor 1, Section 2. The course lasts 3 weeks, from March 25 - April 12, 2019 lectures and practical course will be mainly in the first and second week, starting at 8:15 a.m. with the lecture. The third week will be for evaluation of results, personal work at the instruments, protocol and final report preparation.
Information
Aims, contents and method of the course
The main topic of this electron microscopy course is the detection of heavy metals and other chemical elements in plant- and animal- cells and tissues by means of energy dispersive X-ray microanalysis (EDX). An introduction is also given in electron energy loss spectroscopy (EELS), the use of unstained ultrathin, and of semithin sections in the TEM and SEM. The investigations are made on the filtering TEM Zeiss EM 902 and TEM Zeiss Libra 120, as well as on the digital SEM Philips XL-30 and JEOL JSM IT300. Preparation methods freeze-drying, carbon-coating, ultrathin sectioning by ultramicrotome and contrasting of sections on grids for TEM.
Assessment and permitted materials
Workshop 25%, Lab- and Microscopy work 25%, Protocol 25%, Oral report at final seminar 25%
Minimum requirements and assessment criteria
Presentation of a project on heavy metal contamination and stress of animals and plants. An additional aim is the knowledge of special SEM and TEM-methods and of techniques for sample preparation.
Examination topics
The results of the project- experiments will be discussed and the advanced techniques of electron microscopy will be dealt with , such as EELS, ESI, Contrast Tuning, ESEM, EDX, BSE, Dot-Mapping, semithin and ultrathin sectioning on the Ultramicrotome, advanced SEM and TEM.
Reading list
Will be offered during the course
Association in the course directory
WZB, UF MA BU 04, MZO 2, MEC-9 , MBO 7
Last modified: Th 31.01.2019 09:28