Universität Wien

300190 PP Hands-on Cryotechniques for Immuno-Electron Microscopy and Tissue in-situ Hybridization (2009W)

To enable advanced BIO-stud. to understand & apply advan.cryo-electron microscopy, immunolabelling & tissue in-situ hybridization (TISH) tech. within their own research. Supported by lectures & demonst., this intensive, hand-on training courese teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling or TISH

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Hands-on Cryotechiques in immunoEM and ISH course

All lectures will take place in the Ecology Conference Room
03.11.09 0800-1000 Preliminary lecture
16.11.09 0800-1200 First Lectures
17.11.09 1600-1800 (Student talks)
18.11.09 0800-1000
20.11.09 0800-0900
23.11.09 0800-1000
24.11.09 1600-1800
25.11.09 0800-1000
27.11.09 0800-0900
30.11.09 0800-1000
02.12.09 0800-1000
04.12.09 0800-0900 Final Lecture

Practical sessions will be held in Ultrastructure Research Laboratory, Electron Microscopy Lab in Althanstrasse 14, 1090
16.11.09 0900- 1700 each day for three weeks until 04.12.09 0900-1700 inclusive, when there is no lecture, the practical session may begin earlier, but you will be advised.

Additional practical sessions will be held in Electron Microscopy Lab, Dr.Bohrgasse 9, 1030 and also in Electron Microscopy Lab of IMBA, Dr. Bohrgasse 6, 1030

All students are requested to provide an abstract (max 300 words) describing your major research interest and the intended application of immuno-electron microscopy and/or tissue in-situ hybridization in your research. This abstract must be received by email to Dr. Jacqueline Montanaro and Dr. Siegfried Reipert. Latest acceptance of abstract is 31st October, 2009.

In addition, please provide the following information:
Your Name, Institute, Department, Phone number, email, webpage and please indicate (yes or no) whether you have previous experience with
LRW embedding, FISH, TEM, Epifluorescence microscopy, cryo-microtomy and any immuno-staining techniques at light microscopy or electron microscopy level.

Please send abstracts and further information to:
montanaro@microbial-ecology.net
siegfried.reipert@univie.ac.at

You are advised that you will be required to give a 7 minute introductory talk during the course (17.11.09) describing your major research interest and the intended application of immuno-electron microscopy and/or tissue in-situ hybridization in your research. This is intended to be an introduction of the students to the lecturers and to eachother. This talk will also contribute towards the final assessment.

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 5 participants
Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

Fixierung v. (eigenem) Material mit Hilfe v. Hochdruck Frieren und Gefriersubstitutionstechniken; Einbettung f. feinstrukt. Untersuchungen od. f. d. Immunogoldmakierung; Präparation v. Semi-& Ultradünnschnitten. Einf. in d. Tokuyasu Methode z. Herstellung v. Cryoschnitten für d. Immungoldmarkierung. Gewebe in situ Hybridisierung (TISH) auf Acryl Semidünnschnitten. Evaluation und detaillierte Diskussionen d. Resultate a. Transmission Elektromikroskop & Fluoreszenzmikroskop

Assessment and permitted materials

Minimum requirements and assessment criteria

To enable advanced BIO-stud. to understand & apply advan.cryo-electron microscopy, immunolabelling & tissue in-situ hybridization (TISH) tech. within their own research. Supported by lectures & demonst., this intensive, hand-on training course teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling or TISH

Examination topics

Reading list


Association in the course directory

BZO 11, BZO 13, BOE 10, BOE 11, BOE 12, MZO 2, MEV 2

Last modified: Mo 07.09.2020 15:43