Universität Wien
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300190 PP Hands-on Cryopreparation techniques and Immunolabelling for Light and Electron Microscopy (2013W)

To enable advanced BIO-stud. to understand & apply advan.cryo-electron microscopy, immunolabelling within their own research. Supported by lectures & demonst., this intensive, hand-on training courese teaches special. techn.,such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

NOTE: Students will give a 5 minute Topic of Interest introduction talk including a description of the samples they will use in the course on the 4. October 2013. This talk will be immediately followed by a 5 minute discussion for recommendations on preparation of their samples for the course.
Moodle

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: English

Lecturers

Classes

Currently no class schedule is known.

Information

Aims, contents and method of the course

NOTE: Students will give a 5 minute Topic of Interest introduction talk including a description of the samples they will use in the course. This talk will be immediately followed by a 5 minute discussion for recommendations on preparation of their samples for the course. This will be on 4.10.2013 at 1200-1400 in UZA1, Seminar Room 1.

Hands-on Cryotechniques in immunoEM course; ; All lectures will take place in the Seminar Room 1 UZA I (Zoology Dept.); ; Datum N.U: 04.10.2013 ; Zeit: 1200-1400 Preliminary lecture (Student talks); First Lectures; ; Ort: UZA I Seminar Room 1 ; 11.11.13 0845-1345; 12.11.13 0930-1030 u. 1315-1400; 15.11.13 0900-1100; 18.11.13 0900-1000; 20.11.13 1300-1700; 21.11.13 0900-1045; 22.11.13 1400- 1600 (Round table discussion); 29.11.13 0900-1200 (Students results talks; poster presentation); 29.11.13 1300-1500 (Oral exam); ;
Practical sessions will be held in Ultrastructure Research Laboratory, Electron Microscopy Lab in Althanstrasse 14, 1090; 11.11.13 0900- 1700 each day for three weeks until 29.11.13 0900- 1700 inclusive, when there is no lecture, the practical session may begin earlier, but you will be advised. ; An additional demonstration session will be held in an Electron Microscopy Lab of a different institute, or university in Vienna to expose students to extra techniques, not available at the CIUS core facillity, which are nonetheless available in Vienna (time and date TBA during course).

All students are kindly requested to provide an abstract (max. 200 words) describing your major research interest and the intended application of immuno-electron microscopy in your research. This abstract must be received by email to Dr. Jacqueline Montanaro and Dr. Siegfried Reipert. Latest acceptance of abstract is 14. October, 2013.; ; In addition, please provide the following information:; Your Name, Institute, Department, Phone number, email, webpage and please indicate (yes or no) whether you have previous experience with ; LRW embedding, TEM, Epifluorescence microscopy, ultra cryo-microtomy and any immuno-staining techniques at light microscopy or electron microscopy level.; ; Please send abstracts and further information to:; jacqueline.montanaro@meduniwien.ac.at and siegfried.reipert@univie.ac.at. ; ; You are advised that you will be required to give a 5 minute introductory talk during the course (04.10.13) describing your major research interest and the intended application of immuno- electron microscopy in your research. This is intended to be an introduction of the students to the lecturers and to eachother. This student introduction talk will contribute towards the final assessment.

Assessment and permitted materials

Student assessment is divided into 4 parts:
(1) Introductory Student talk (5 mins) and abstract, 4.10.2013
(2) Participation in lectures, group poster preparation and practicum,
(3) Individual oral assessment of understanding technical and theoretical knowledge presented in lectures and demonstrations (end of course),
(4) Final student talk (7-10 mins) followed by open discussion and questions.

Minimum requirements and assessment criteria

To enable advanced BIO-stud. to understand & apply advan.cryo preparation techniques to electron microscopy (EM), using immunolabelling within their own research. Supported by lectures & demonst., this intensive, hand-on training course teaches special. techn., such as Tokuyasu cryosectioning, high-pressure freezing, freeze substitution & acrylic resin embedding for high resol. localization of intracell. proteins & other intracell. struc. by immunogold labelling. Plunge freezing and freeze drying for scanning EM will be demonstrated.

Examination topics

Hands-on Cryo preparation techniques and Immunolabelling for Light and Electron Microscopy - To enable advanced BIO-stud. to understand & apply advan.cryopreparation techniques for light and electron microscopy, using immunolabelling. within their own research. Supported by lectures & demonst., this intensive, hand-on training course teaches special techniques, such as Tokuyasu cryosectioning, high-pressure freezing-freeze substitution & acrylic resin embedding for high resol. localization of intracellular proteins & structures.

Reading list

Association in the course directory

MBO 7, MMB W-2, MMI III-2, MGE III-2, MZO 2, MEV 2, MPF W-2

Last modified: Mo 07.09.2020 15:43