Universität Wien FIND

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300218 UE Scanning electron microscopy - techniques and preparations for biologist (2017S)

4.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Contact me in advance! (daniela.gruber@univie.ac.at)

Compulsory attendance for preliminary meeting, the safety instructions and the 6 days of course. The attendance of this course is mandatory for all students who want to use our scanning electron microscopes.

Registration/Deregistration

Details

max. 5 participants
Language: German, English

Lecturers

Classes

Fr. 03.03.2017 08:15 - 09:00 mandatory preliminary meeting, 09:15 - 10:30 mandatory safety instructions; Konferenzraum Zoologie, afterwardss EM Lab
Mo. 06.03.2017 - 12:00 lecture Konferenzraum Zoologie
13:00 - 16:00 practical work, EM-LAB Core Facility Cell Imaging and ultrastructure research
Di. 07.03.2017 8:15 - 16:00 practical work, EM-LAB Core Facility Cell Imaging and ultrastructure resarch
Mi. 08.03.2017 8:15 - 16:00 practical work, EM-LAB Core Facility Cell Imaging and ultrastructure resarch
Do. 09.03.2017 8:15 - 16:00 practical work, EM-LAB Core Facility Cell Imaging and ultrastructure resarch
Fr. 10.13.2017 8:15 - 16:00 practical work, EM-LAB Core Facility Cell Imaging and ultrastructure resarch
Mo. 13.03.2017 8:15- 12:00 final discussion, examination, Konferenzraum Zoologie


Information

Aims, contents and method of the course

Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)

Assessment and permitted materials

cooperation: 40%
oral exam: 30% (13.03.2017)
protocol: 30% (date of submission: 24.03.2017)

each part has to be positive

Minimum requirements and assessment criteria

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be offered during the course.

Association in the course directory

MZO W-1, WZB, M-WZB, MAN 3, MEC-9, MBO 7

Last modified: Mo 07.09.2020 15:43