300218 UE Scanning electron microscopy - techniques and preparations for biologists (2018S)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Fr 09.02.2018 08:00 to Th 22.02.2018 18:00
- Deregistration possible until Sa 31.03.2018 18:00
Details
max. 6 participants
Language: German, English
Lecturers
Classes
Preliminary meeting and allocation of free places (mandatory attendance): Thursday, 01.03.2018; 09:30 – 11:00 (Seminar Room 1)
Safety instructions and the lecture Basics of the Scanning Electron microscope: Friday, 02.03.2018; 8:15 – 15:00 (Seminar Room 1)
Debriefing, presentation of results: Thuesday, 13.03.2018, 8:15 – 13:00 (Seminar Room 1)
This course is mandatory for all students who want to use our scanning electron microscopes.
Information
Aims, contents and method of the course
Assessment and permitted materials
cooperation: 40%
oral presentation of results: 40% (13.03.2018)
protocol: 20% (date of submission: 23.03.2018)each part has to be positive
oral presentation of results: 40% (13.03.2018)
protocol: 20% (date of submission: 23.03.2018)each part has to be positive
Minimum requirements and assessment criteria
Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
Examination topics
Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.
Reading list
Will be presented during course.
Association in the course directory
MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7
Last modified: Mo 07.09.2020 15:43
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)