Universität Wien
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300218 UE Scanning electron microscopy - techniques and preparations for biologists (2018S)

4.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).


max. 6 participants
Language: German, English



Preliminary meeting and allocation of free places (mandatory attendance): Thursday, 01.03.2018; 09:30 – 11:00 (Seminar Room 1)
Safety instructions and the lecture Basics of the Scanning Electron microscope: Friday, 02.03.2018; 8:15 – 15:00 (Seminar Room 1)

Practical work: Monday, 05.03.2018 – Monday, 12.03.2018; 8:15 – 15:00 (EM-Lab CIUS
Debriefing, presentation of results: Thuesday, 13.03.2018, 8:15 – 13:00 (Seminar Room 1)
This course is mandatory for all students who want to use our scanning electron microscopes.


Aims, contents and method of the course

Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)

Assessment and permitted materials

cooperation: 40%
oral presentation of results: 40% (13.03.2018)
protocol: 20% (date of submission: 23.03.2018)

each part has to be positive

Minimum requirements and assessment criteria

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be presented during course.

Association in the course directory

MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7

Last modified: Mo 07.09.2020 15:43