300218 UE Scanning electron microscopy - techniques and preparations for biologists (2019S)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Fr 08.02.2019 08:00 to Th 21.02.2019 18:00
- Deregistration possible until Su 31.03.2019 18:00
Details
max. 6 participants
Language: German, English
Lecturers
Classes (iCal) - next class is marked with N
ATTENTION: No online registration possible.
Personal registration and agreement on the subject of the course not later than 21.02.2019: daniela.gruber@univie.ac.at, room 1032, level 1.The final allocation will take place on Monday, 01.03.2019 punctually at 8:15 am directly before the obligatory safety instruction!Maximum number of participants: 6Program:Final allocation and safety instruction: Friday 01.03.2019 08:15 - 10:00 Conference room Zoology, UZA 1, Biozentrum Althanstraße 14, 1.015 EG 1.015Introductory short lectures of the students and theory: Monday 04.03.2019 08:15 - 15:00 Conference Room of Zoology, UZA 1, Biozentrum Althanstraße 14, 1.015 EG 1.015
Final meeting and short presentations: Wednesday 13.03.2019 08:15 - 13:00 Conference Room of Zoology, UZA 1, Biozentrum Althanstraße 14, 1.015 EG 1.015Practical part:
DI 05.03.2019 - DI 12.03.2019 from 8:15 - 3:00 pm in the EM Laboratory, Cell Imaging and Ultrastructure Research, UZA 1, Biozentrum Althanstraße 14, 1090 Wien. 1 . level
Friday
01.03.
08:00 - 10:00
Konferenzraum
Monday
04.03.
08:15 - 15:00
Konferenzraum
Wednesday
13.03.
08:15 - 13:00
Konferenzraum
Information
Aims, contents and method of the course
Assessment and permitted materials
1) Short presentation (about 5 minutes): For which scientific question should the Scanning Electron Microscope be used? Presentation of the sample to be prepared and examined during the course (25%)
2) Cooperation (40%)
3) Short presentation (5 - 10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (25%)
4) Protocol 10%Every part has to be positive
2) Cooperation (40%)
3) Short presentation (5 - 10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (25%)
4) Protocol 10%Every part has to be positive
Minimum requirements and assessment criteria
Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
Examination topics
Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.
Reading list
Will be announced during the course.
Association in the course directory
MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2
Last modified: Sa 22.10.2022 00:29
After successful completion of the course, the corresponding equipment may be used immediately independently and on its own responsibility.
Participation in this course without a specific question and without sample material is only possible if there are still places available.Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)