300218 UE Scanning electron microscopy - techniques and preparations for biologists (2019W)
Continuous assessment of course work
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max. 6 participants
Language: German, English
Lecturers
Classes (iCal) - next class is marked with N
ATTENTION: No online registration possible.
Personal registration and agreement on the subject of the course from now on until 13.09.2019: daniela.gruber@univie.ac.at, room 1032, level 1.The final confirmation and allocation of places 01.10.2019 punctually at 8:00 am directly before the obligatory safety instruction!Maximum number of participants: 6Program:01.10.2019
08:15-09:30 Safety instructions together with TEM course
09:30 - 10:30 Short presentations of students (planned work during the course)
10:30 - 15:00 Lecture part 1
02.10.2019
08:15 - 12:00 Lecture part 2
13:00 - 15:00 Preparation of chemicals
03.10.2019
08:15 - 15:00 Fixation, dehydration of samples, introduction SEM JEOL IT 300
04.10.2019
08:15 - 15:00 Critical point drying, mounting, gold / carbon coating, Introduction SEM Philips XL 30 ESEM
07.10.2019
08:00 - 15:00 Examination of own samples
08.10.2019
08:15 -12:00 Introduction Low Vac technique
12:00 - 15:00 Examination of own samples (both SEMs)
09.10.2019
08:15 - 12:00 Introduction EDX (energy dispersive X-ray spectroscopy) SEM JEOL IT 300
12:00 - 15:00 Examination of own samples (both SEMs)
10.10.2019
09:00 - 12:00 Demonstration Cryotransfer (SEM JEOL IT 300)
13:00 - 15:00 Preparation time for short presentations, final meeting and protocol
11.10.2019
08:15 -12:00 Short presentations, final meeting
- Tuesday 01.10. 08:15 - 15:00 Konferenzraum
- Wednesday 02.10. 08:15 - 12:15 Konferenzraum
- Friday 11.10. 08:15 - 12:00 Übungsraum 4
Information
Aims, contents and method of the course
Assessment and permitted materials
1) Short presentation (about 5 minutes): For which scientific question should the Scanning Electron Microscope be used? Presentation of the sample to be prepared and examined during the course (25%)
2) Cooperation (40%)
3) Short presentation (5 - 10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (25%)
4) Protocol 10%Every part has to be positive
2) Cooperation (40%)
3) Short presentation (5 - 10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (25%)
4) Protocol 10%Every part has to be positive
Minimum requirements and assessment criteria
Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
Examination topics
Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.
Reading list
Is presented during the course
Association in the course directory
MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2
Last modified: Sa 22.10.2022 00:29
After successful completion of the course, the corresponding equipment may be used immediately independently and on its own responsibility.
Participation in this course without a specific question and without sample material is only possible if there are still places available.Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)