Universität Wien FIND

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300218 UE Scanning electron microscopy - techniques and preparations for biologists (2019W)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


max. 6 participants
Language: German, English


Classes (iCal) - next class is marked with N

ATTENTION: No online registration possible.

Personal registration and agreement on the subject of the course from now on until 13.09.2019: daniela.gruber@univie.ac.at, room 1032, level 1.

The final confirmation and allocation of places 01.10.2019 punctually at 8:00 am directly before the obligatory safety instruction!

Maximum number of participants: 6

08:15-09:30 Safety instructions together with TEM course
09:30 - 10:30 Short presentations of students (planned work during the course)
10:30 - 15:00 Lecture part 1
08:15 - 12:00 Lecture part 2
13:00 - 15:00 Preparation of chemicals
08:15 - 15:00 Fixation, dehydration of samples, introduction SEM JEOL IT 300
08:15 - 15:00 Critical point drying, mounting, gold / carbon coating, Introduction SEM Philips XL 30 ESEM
08:00 - 15:00 Examination of own samples
08:15 -12:00 Introduction Low Vac technique
12:00 - 15:00 Examination of own samples (both SEMs)
08:15 - 12:00 Introduction EDX (energy dispersive X-ray spectroscopy) SEM JEOL IT 300
12:00 - 15:00 Examination of own samples (both SEMs)
09:00 - 12:00 Demonstration Cryotransfer (SEM JEOL IT 300)
13:00 - 15:00 Preparation time for short presentations, final meeting and protocol
08:15 -12:00 Short presentations, final meeting

Tuesday 01.10. 08:15 - 15:00 Konferenzraum der Zoologie, UZA 1,Biozentrum Althanstraße 14, 1.015 EG
Wednesday 02.10. 08:15 - 12:15 Konferenzraum der Zoologie, UZA 1,Biozentrum Althanstraße 14, 1.015 EG
Friday 11.10. 08:15 - 12:00 Übungsraum 4 UZA 1, Biozentrum Althanstraße 14, Morphologie Z2.016 1.OG


Aims, contents and method of the course

This course is intended for those colleagues who are planning to use one of the Scanning Electron Microscopes in the EM Lab(Core Facility Cell Imaging and Ultrastructure Research) as part of their Master Thesis or PhD work and who already have a specific research question and a sample to be worked on during the course.
After successful completion of the course, the corresponding equipment may be used immediately independently and on its own responsibility.
Participation in this course without a specific question and without sample material is only possible if there are still places available.

Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)

Assessment and permitted materials

1) Short presentation (about 5 minutes): For which scientific question should the Scanning Electron Microscope be used? Presentation of the sample to be prepared and examined during the course (25%)
2) Cooperation (40%)
3) Short presentation (5 - 10 minutes): Which results were achieved? Where do possibilities arise to improve the results? What would a further experiment planning look like? (25%)
4) Protocol 10%

Every part has to be positive

Minimum requirements and assessment criteria

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Is presented during the course

Association in the course directory

MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2

Last modified: Mo 07.09.2020 15:22