Universität Wien FIND
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300218 UE Scanning electron microscopy - techniques and preparations for biologists (2020W)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).


max. 6 participants
Language: German, English



Attention: Due to the limited space in our lab rooms this course will be held as hybrid teaching. All meetings, the lectures, exams... will be digital. For each of the 6 participants one day is reserved to work hands on our instruments. The mandatory preliminary meeting will be on 1.10.2020 8:00. I will provide al link for all participants.
In this meeting the time slots for the lab work will be allocated. All mandatory online meetings and the practical work in the lab will be 01.10.2020 – 14.10.2020. The 6 days of presence each for one student will be 05.10 – 12.10.2020, 08:00 – 14:00.


Aims, contents and method of the course

Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold and carbon, cryopreparation.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector (demo), cryo transfer of deep frozen samples (DEMO)

Assessment and permitted materials

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.

Minimum requirements and assessment criteria

1) Cooperation (50%)
2) Protocol (25%)
4) Questionnaires (25%)

Every part has to be positive

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be presented during the course

Association in the course directory

MZO W-1, M-WZB, MAN 3, MEC-9, MBO 7, MZO 2

Last modified: Fr 04.09.2020 06:29