Universität Wien FIND

300218 UE Scanning electron microscopy - techniques and preparations for biologists (2021W)

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).


max. 6 participants
Language: German, English


Classes (iCal) - next class is marked with N

The practical part of the course will take place from December 9th, 2021 - Dezember 17th, 21st in the Laboratory for Electron Microscopy.

Monday 06.12. 08:00 - 14:45 Digital


Aims, contents and method of the course

Contents and methods:
Our Scanning Electron Microscopes (SEM) offer various techniques to investigate samples.
In this course you will have the opportunity to learn the necessary preparation steps and the usage of the SEM in several modes so that you can apply these techniques with your own samples afterwards.
The following techniques will be offered for sample preparation:
Fixation (glutaraldehyde, OsO4), dehydration (Ethanol, Acetone, DMP), drying (critical point, chemical), coating with gold.
On the SEM you will use the following modes:
Secondary electron detector in low vacuum and high vacuum, backscatter electron detector in low vacuum and high vacuum, energy dispersive x-ray micro analysis with EDX detector.

Assessment and permitted materials

Choice of relevant methods for sample preparation, practical work in our chemlab, operating preparation instruments and SEM, evaluation and interpretation of results.
1) Cooperation
2) Short Presentation
3) Worksheets

Minimum requirements and assessment criteria

Every part has to be positive
Cooperation 50 points
Short presentation 25 points
Worksheets 25 points

0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1

Examination topics

Theoretical background (lecture). Operating the preparation instruments and the SEM (practical work). Evaluation and interpretation of the results.

Reading list

Will be presented during the course

Association in the course directory

M-WZB, MAN 3, MEC-9, MBO 7, MZO 2, MZO4

Last modified: Th 23.03.2023 00:25