300221 UE Transmission Electron Microscopy of Cells and Tissues -Techniques and Preparation (2018W)
Labels
Registration/Deregistration
- Registration is open from Th 06.09.2018 08:00 to Th 20.09.2018 18:00
- Deregistration possible until We 31.10.2018 18:00
Details
Lecturers
Classes
Friday. 5.10.2018. 08:15- 10:00 Compulsory safety instruction by Daniela Gruber; Conference room 1.015, UZA1, Bio-center Althanstr. 14, 1090 Vienna
Wednesday, 17.10.2018. 09:00 – 12:00 Lecture SR 1, 13:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Thursday. 18.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Friday. 19.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Monday. 22.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Wednesday. 24.10.2018. 9:00-12: Lecture SR 1; 13:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Thursday. 25.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Monday. 29.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Wednesday. 31.10.2018. 8:15 – 12:00. Final discussion, talks, Übungsraum 3
Information
Aims, contents and method of the course
Assessment and permitted materials
Oral presentation of the results: 40% (23th March 2018)
Protocol: 20%
Every item has to be positive.
The following preparation steps will be exercised in practice: Chemical fixation (glutaraldehyde, paraformaldehyde, OsO4), dehydration, embedding in epoxy resin, thin-and ultrathin sectioning with ultramicrotomes, contrasting of sections, evaluation and interpretation of the results in the TEM.
A preview will be given concerning advanced sample preparation techniques based on cryotechniques, which all build up on basic skills trained during our course.