Universität Wien FIND
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300221 UE Transmission Electron Microscopy of Cells and Tissues -Techniques and Preparation (2018W)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 6 participants
Language: German, English

Lecturers

Classes

Friday. 5.10.2018. 08:15- 10:00 Compulsory safety instruction by Daniela Gruber; Conference room 1.015, UZA1, Bio-center Althanstr. 14, 1090 Vienna
Wednesday, 17.10.2018. 09:00 – 12:00 Lecture SR 1, 13:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Thursday. 18.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Friday. 19.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Monday. 22.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Wednesday. 24.10.2018. 9:00-12: Lecture SR 1; 13:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Thursday. 25.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Monday. 29.10.2018. 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Wednesday. 31.10.2018. 8:15 – 12:00. Final discussion, talks, Übungsraum 3


Information

Aims, contents and method of the course

Transmission Electron Microscopy (TEM) has been proven as a valuable tool for a variety of research topics in life sciences. This course provides skills in sample preparation and the use of TEMs. It should enable students to work independently with their own samples.
The following preparation steps will be exercised in practice: Chemical fixation (glutaraldehyde, paraformaldehyde, OsO4), dehydration, embedding in epoxy resin, thin-and ultrathin sectioning with ultramicrotomes, contrasting of sections, evaluation and interpretation of the results in the TEM.
A preview will be given concerning advanced sample preparation techniques based on cryotechniques, which all build up on basic skills trained during our course.

Assessment and permitted materials

Participation: 40%
Oral presentation of the results: 40% (23th March 2018)
Protocol: 20%
Every item has to be positive.

Minimum requirements and assessment criteria

Theoretical knowledge (lecture). Application of equipment for sample preparation and TEMs (practical work). Evaluation, discussion and interpretation of the results.

Examination topics

Ability to select suitable methods for TEM sample preparation, practical work in our chemistry lab, handling of equipment for sample preparation incl. ultrathin sectioning with ultramicrotomes, work with TEMs, interpretation and evaluation of the results.

Reading list

M. A. Hayat (2000). Principles and Techniques of Electron Microscopy- Biological Applications. 4th Edition, Cambridge University Press, isbn: 9780521632874

Association in the course directory

MZO W-1, M-WZB

Last modified: Tu 04.12.2018 14:08