Universität Wien FIND
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300221 UE Transmission Electron Microscopy of Cells and Tissues -Techniques and Preparation (2019S)

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 6 participants
Language: German, English

Lecturers

Classes

Attention! Application via online system is not possible.
This course, preferentially, aims on students who want to answer specific questions by using transmission electron microscopy at the CIUS as part of their MSc- or PhD thesis. Moreover, other interested students might apply for the course with a maximum number of 6 participants.
Students interested in the course are asked to contact Siegfried Reipert until 20th February 2019 personally (for appointment email: siegfried.reipert@univie.ac.at; Althanstr. 14 UZA 1, room 1.111).

The participation in the safety instruction and in the 8 course days is obligatory. The participation is precondition for the use of our transmission electron microscope and preparation equipment.

Fr. 1.03.2019: 8:00 -10:00 Safety instructions; conference room Zoology , UZA 1, Althanstraße 14, 1090 Vienna , (together with course 300218 UE)

We. 13.03.2019 09:00 – 12:00 lecture (conference room zoology), 13:00 – 16:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Th. 14.03.2019 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Fr. 15.03.2019 9:00 – 13:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Mo. 18.03.2019 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Tu. 19.03.2018 9:00– 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
We. 20.03.2018 9:00 – 12:00: lecture SR1; 13:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Th. 21.03.2018 9:00 – 17:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Fr. 22.03.2018 9:00 – 13:00 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research
Mo. 25.03.2018 9:00 – 13:00 presentation of the results, final discussion, SR1


Information

Aims, contents and method of the course

Transmission Electron Microscopy (TEM) has been proven as a valuable tool for a variety of research topics in life sciences. This course provides skills in sample preparation and the use of TEMs. It should enable students to work independently with their own samples.
The following preparation steps will be exercised in practice: Chemical fixation (glutaraldehyde, paraformaldehyde, OsO4), dehydration, embedding in epoxy resin, thin-and ultrathin sectioning with ultramicrotomes, contrasting of sections, evaluation and interpretation of the results in the TEM.
A preview will be given concerning advanced sample preparation techniques based on cryotechniques, which all build up on basic skills trained during our course.

Assessment and permitted materials

Participation: 40%
Oral presentation of the results: 40% (25th March 2019)
Protocol: 20%
Every item has to be positive.

Minimum requirements and assessment criteria

Theoretical knowledge (lecture). Application of equipment for sample preparation and TEMs (practical work). Evaluation, discussion and interpretation of the results.

Examination topics

Ability to select suitable methods for TEM sample preparation, practical work in our chemistry lab, handling of equipment for sample preparation incl. ultrathin sectioning with ultramicrotomes, work with TEMs, interpretation and evaluation of the results.

Reading list

M. A. Hayat (2000). Principles and Techniques of Electron Microscopy- Biological Applications. 4th Edition, Cambridge University Press, isbn: 9780521632874

Association in the course directory

MZO W-1, M-WZB, MZO 2

Last modified: We 06.02.2019 11:28