Universität Wien FIND

300234 VO Biochemical and biophysical characterization and validation approaches (2016S)

3.00 ECTS (2.00 SWS), SPL 30 - Biologie

Registration/Deregistration

Details

Language: English

Examination dates

Lecturers

Classes

NB: This year the course is offered ONLY as a one-week block: the lectures will takes place ONLY on the week April 5-13th in the morning (09:00-10:30 and 10:45-12:15). There will be NO possibility to accommodate the lectures on different dates.


Information

Aims, contents and method of the course

This theoretical course will start with introductory lectures on the basic concepts in structural biology and cover modern approaches and fundamentals of the methods used in recombinant protein expression, purification, biophysical characterization and quality assessment of macromolecular samples as well as cell biophysics approaches. The covered topics include static and dynamic light scattering, fundamentals of X-ray crystallography, absorption and fluorescence spectroscopy techniques (CD, FRET, FLIM, FC(C)S, fluorescence anisotropy, etc), mass spectroscopy and imaging methods (light and electron microscopy).

Assessment and permitted materials

Verpflichtende Anwesenheit. Die Beurteilung basiert auf folgenden Teilleistungen: Qualität und Aufbereitung der Seminarpräsentation, aktive Teilnahme an den Diskussionen der vorgestellten Publikationen, schriftliche Beiträge (die prozentuelle Aufteilung der Teilleistungen wird vom Kursleiter bekanntgegeben). Jede der Teilleistungen muss positiv sein.

Minimum requirements and assessment criteria

Provide a comprehensive overview of strategies and methods for protein expression and purification; enable students to choose a better strategy for a given protein
Highlight the utility of different methods of protein characterization for understanding its structure and function using specific examples
Explain theoretical background and instrumentation used for protein characterization in amount sufficient for critical interpretation of original research results
Enable students to choose an adequate method for a specific question

Examination topics

Reading list


Association in the course directory

PhD MB 2, MMB I-2, MMB II-2, M-WZB, MGE III-1,MMEI III, MMB W-2

Last modified: Th 05.09.2019 12:58