Universität Wien FIND

300246 UE Practical Course in Molecular Microbiology, Microbial Ecology and Immunobiology 1 (2016S)

Molecular Microbiology

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


max. 8 participants
Language: English



Vorbespr: 8.März 2016, 19.00 Uhr
BZB/SR 3, Dr.Bohrgasse 9,1030 Wien.
Voraussetzung ist die VO Pathogene Bakterien im WS !
Ort der Übungen:VetMed 1210 Wien,
Termin wird bei Vorbesprechung vereinbart.(voraussichtl. September)
Für externe Studierende: Zulassungsvoraussetzungen für diese Übungen sind prüfungsimmanente Lehrveranstaltungen (UE) der Auflagen zur Studienzulassung.
For external students: requirements for this practical course are the completion of compulsory courses with continuous assessment (UE) from the admission certificate.
Weitere Infos: http://molekularebiologie.univie.ac.at/


Aims, contents and method of the course

In this course the students have to classify and to identify unknown bacterial isolates which are subjected to a set of different methods nowadays applied in bacterial systematics. These methods include a) classical approaches such as Gram-staining, tests for Oxidase and Katalase, utilisation of carbon sources and production of acid from sugars; b) chemotaxonomic methods such as analysis of quinones, polar lipids, mycolic acids, cell wall diamino acids and polyamines; c) DNA-based methods including generation of genomic fingerprints after ERIC-, REP- and BOX-PCR, restriction analysis of amplified ribosomal DNA (ARDRA) and comparison of sizes of DNA amplicons obtained after amplification of the 16S-23S interspacers (ITS). Based on the results the bacterial isolates are classified in comparison with data from literature and allocated to genera or species.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Examination topics

Reading list

Association in the course directory


Last modified: Fr 31.08.2018 08:56