300269 PP Fluorescence in situ hybridization (FISH) (2015S)
Identification of uncultured microorganisms
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 05.02.2015 08:00 to Th 19.02.2015 18:00
- Deregistration possible until Fr 27.03.2015 18:00
Details
max. 12 participants
Language: German
Lecturers
Classes
Blockveranstaltung vom 14.-25.9.2015, ÜR7 und Seminarraum Mikrobielle Ökologie.
Information
Aims, contents and method of the course
In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Cooperation during the course, written protocol, seminar presentation, written exam.
Minimum requirements and assessment criteria
Acquisition of methods to extract DNA from environmental samples, to PCR-amplify and clone specific genes, and of FISH and fluorescence microscopy. Use of these methods on participants¿ own environmental samples and evaluation of the results.
Examination topics
Molecular biological methods (DNA extraction, PCR, cloning, FISH), microscopy, digital image analysis.
Reading list
Association in the course directory
B-BOE 12, B-BOE 13, B-BMG 12A, B-BMB 11A, MMI I-2, MGE III-1
Last modified: Mo 07.09.2020 15:43