Universität Wien FIND

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300286 PP Fluorescence in situ hybridization (FISH) (2016W)

Identification of uncultured microorganisms

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work



max. 12 participants
Language: German



Beginn: Vorbesprechung am 5.10.2016 um 14 Uhr im Seminarraum Mikrobielle Ökologie (2.309). Die Anwesenheit bei der Vorbesprechung ist Voraussetzung für die Teilnahme an der Lehrveranstaltung.

Block vom 13.-24.2.2017, 9-18 Uhr (Übungsraum 7 + Seminarraum DOME)

Hinweis: Dieses Praktikumsangebot richtet sich präferentiell an Bachelorstudenten, sowie an Masterstudenten mit mikrobiologischem Interesse.


Aims, contents and method of the course

In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.

Assessment and permitted materials

Continuous assessment of course work. Participation during the course, written protocol, seminar presentation, written exam.

Minimum requirements and assessment criteria

Active participation in lab experiments, discussions, and seminar presentation (40%);
Written exam and protocol (60%)

Examination topics

Content of lectures during the course and content of course hand book

Reading list

Association in the course directory

B-BOE 12, B-BOE 13, B-BMG 12A, B-BMB 11A, MGE III-1, BOE 12, BOE 13

Last modified: Mo 07.09.2020 15:43