300286 UE Fluorescence in situ hybridization (FISH) (2018W)
Identification of uncultured microorganisms
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 06.09.2018 08:00 to Mo 31.12.2018 18:00
- Deregistration possible until Th 31.01.2019 18:00
Details
max. 12 participants
Language: German
Lecturers
Classes
Blockveranstaltung: 18.2.2019 - 1.3.2019, Übungsraum 7 (Anatomie) + Seminarraum DoME
Information
Aims, contents and method of the course
In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Participation during the course, written protocol, seminar presentation, written exam.
Minimum requirements and assessment criteria
Active participation in lab experiments, discussions, and seminar presentation (40%);
Written exam and protocol (60%)
Written exam and protocol (60%)
Examination topics
Content of lectures during the course and content of course hand book
Reading list
Association in the course directory
BOE 12
Last modified: Mo 18.02.2019 14:49