300286 UE Fluorescence in situ hybridization (FISH) (2023W)
Identification of uncultured microorganisms
Continuous assessment of course work
Labels
Für diese LV wird eine verpflichtende Sicherheitsunterweisung stattfinden. Nach Ablauf der Anmeldephase und Aufnahme der Studierenden, können die entsprechenden Module (Inhalte und Prüfung) über Moodle geöffnet und absolviert werden. Näheres erfahren Sie unter https://laborlehre-ubb.univie.ac.at/fuer-studierende/Alle Übungsräume dürfen ausschließlich mit Labormantel und geschlossenen Schuhen betreten werden, ebenso muss eine Laborbrille angeschafft werden - Privatsachen bitte in den Garderoberäumen verstauen.There will be a mandatory safty instruction for this course. After the registration period, the admitted students can open and complete the respective modules (content and exam) via Moodle.You can find out more at https://laborlehre-ubb.univie.ac.at/fuer-studierende/All practice rooms may only be entered with a lab coat and closed shoes, and lab glasses must also be purchased - please store private belongings in the cloakroom.
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Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 07.09.2023 14:00 to Th 21.09.2023 18:00
- Deregistration possible until Su 15.10.2023 18:00
Details
max. 12 participants
Language: German
Lecturers
Classes (iCal) - next class is marked with N
Blocked course: 5.2. - 16.2.2024
Monday
05.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Tuesday
06.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Wednesday
07.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Thursday
08.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Friday
09.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Monday
12.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Tuesday
13.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Wednesday
14.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Thursday
15.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Friday
16.02.
08:00 - 18:15
Übungsraum 1.8 Molekularbiologie 2, Biologie Djerassiplatz 1, 1.040, Ebene 1
Information
Aims, contents and method of the course
In this laboratory course, prokaryotes in environmental and medical samples will be detected and identified using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.
Assessment and permitted materials
Continuous assessment of course work. Participation during the course, written protocol, seminar presentation, written exam.
Minimum requirements and assessment criteria
Active participation in lab experiments, discussions, and seminar presentation (40%);
Written exam and protocol (60%)
Written exam and protocol (60%)
Examination topics
Content of lectures during the course and content of course hand book
Reading list
Association in the course directory
BOE 12, BMB 10, BMG 10
Last modified: We 13.09.2023 13:48