Universität Wien

300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2018W)

techniques and preparations for biologists

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 5 participants
Language: English

Lecturers

Classes

First date: Thursday 25 .10.2018; 13.30 pm, Seminar Room 1, UZA1. Meeting is compulsory, but students can also send representative in their place.

Summary: Block course 05.11.2018 until 14.11.2018 inclusive; (Attention: the laboratory is not available on 12.11.2018, therefore no course will take place on this day); plus one extra evening meeting.

Details Block course: 5-14 November 2018 (except 12.11.2018); inclusive 08.30-16:30 each day
Mo. 05.11.2018 08:30 – 12:30 Seminar Room 1 UZA1, 13:30 – 16:30 practical work, EM-LAB Core Facility Cell Imaging and Ultrastructure Research. Practical work continues in EM lab.
Results discussion Tuesday 13th November, 08:30-12:00 Seminar Room 1, UZA1
Note: approx. 2 weeks after last course day, allow for additional evening meeting for group oral exam; student presentations; exact day to be arranged at preliminary meeting.

Questions: please send email to jacqueline.montanaro@univie.ac.at


Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light and electron microscopy level, including how to prepare semi-thin and ultrathin sections using a Cryoultramicrotome (Tokuyasu method). Immunolabelling and contrasting using negative staining, cryosections and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed.

Assessment and permitted materials

Participation: 40
Oral Exam: 30
Protocol or Poster: 30
Performance in each part must be positive

Minimum requirements and assessment criteria

as above

Examination topics

Knowledge of the theoretical background (lectures). Application of the technical aspects of immunolabeling for LM and TEM (from the practical part). Applications in examples. Analysis, interpretation and evaluation of results.

Reading list

Wird während des Praktikums bekannt gegeben

Association in the course directory

MZO W-1, MBO 7, MMB W-2, MGE III-2,

Last modified: Fr 31.08.2018 08:43