300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2021W)

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light (LM) and electron microscopy (EM) levels, and how to prepare semi-thin and ultrathin sections using an ultramicrotome. Advanced methods (Tokuyasu method) at the ultrastructural level will also be discussed. Immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Students participating in this course will gain a good understanding of immunological methods for LM and transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret results of immunolabelling experiments.