Universität Wien

300306 UE Hands-on Immunolabelling for Light and Electron Microscopy (2022W)

techniques and preparations for biologists

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).

Details

max. 6 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

The first lecture for the practical course will take place on 11/11/2022 from 13:15 - 14:45 (online or on-site, depending on the Covid situation).

Please note: this is an in-person block practical course with attendance required for 2 weeks; 14.11.2022 - 25.11.2022 inclusive. Preliminary lecture on Friday 11.11.2022 and all lectures on Monday 14.11.2022 are compulsory. Practical sessions will be held in Electron Microscopy laboratory (Level 3, Biologie Djerassiplatz 1) from 9:00 - 17:00. Oral exam and presentations are scheduled on 15.12.2022.

  • Friday 11.11. 13:15 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Monday 14.11. 09:45 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Monday 21.11. 09:45 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Thursday 24.11. 09:45 - 13:00 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Thursday 15.12. 18:30 - 21:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1

Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform immunolabelling at light (LM) and electron microscopy (EM) levels, and how to prepare semi-thin and ultrathin sections using an ultramicrotome. Advanced methods (Tokuyasu method) at the ultrastructural level will also be discussed. Immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared using the fluorescence and transmission electron microscopes. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Students participating in this course will gain a good understanding of immunological methods for LM and transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret results of immunolabelling experiments.

Assessment and permitted materials

Each part must be positive:
Safety training: 15 points
Cooperation: 20 points
Short presentation: 20 points
Detailed protocol or poster: 25 points
Oral examination: 20 points

0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1

Minimum requirements and assessment criteria

as above

Examination topics

Knowledge of the theoretical background (lectures); Safety in the electron microscopy lab. Understanding of different preparation methods. Applications of the technical aspects of immunolabeling for LM and TEM (from the practical part). Analysis, interpretation and evaluation of results.

Reading list

Will be announced during the practical course

Association in the course directory

MZO W-1, MZO4, MBO 7, MMB W-2, MGE III-2,

Last modified: Mo 23.01.2023 14:09