Universität Wien
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300306 UE Transmission Electron Microscopy: A hands-on course including immunolabelling of cells and tissues (2024W)

Hands-on Immunolabelling for Light and Electron Microscopy

5.00 ECTS (3.00 SWS), SPL 30 - Biologie
Continuous assessment of course work
Fr 15.11. 13:15-14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1

Registration/Deregistration

Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
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Details

max. 8 participants
Language: English

Lecturers

Classes (iCal) - next class is marked with N

The course language is English. Compulsory are all the lectures in Seminar room 1.8 (listed above), starting with Friday 15.11.2024 and also the excursion to ISTA campus on 29.11.2024. The practical sessions are designed to be modular in a 2 week block. To accommodate all students with needed essential equipment, each of 5 x half day practical modules will be offered twice. For assignment of students to sessions, more information will be given on Friday 15.11 and choices will be final on Monday 18.11.24. Approx. 2 weeks after the final day of the block course, the oral exams and also presentation of students poster or protocol will be held. The final date will be discussed to suit all course-participating students. If you have any further questions, please contact the course leader, jacqueline.montanaro(at)univie.ac.at

  • Monday 18.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Wednesday 20.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Friday 22.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
  • Monday 25.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1

Information

Aims, contents and method of the course

The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform routine electron microscopy (EM), including how to prepare semi-thin and ultrathin sections using an ultramicrotome and an introduction to the transmission electron microscope. In addition, immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Advanced methods for sample preparation and other label detection methods will be discussed. Students participating in this course will gain a good understanding of routine and immunological methods for transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret both routine preparations for transmission electron microscopy and how to interpret results of immunolabelling experiments.

Assessment and permitted materials

Each part must be positive:
Safety training: 10 points
Cooperation: 20 points
Short presentation: 25 points
Detailed protocol or poster: 25 points
Oral examination: 20 points

0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1

Minimum requirements and assessment criteria

as above

Examination topics

Knowledge of the theoretical background (lectures); Safety in the electron microscopy lab. Understanding of different preparation methods. Applications of the technical aspects of immunolabeling for LM and TEM (from the practical part). Analysis, interpretation and evaluation of results.

Reading list

Will be announced during the practical course

Association in the course directory

MZO4, MBO 7, MEC-9, MZO2, CoBeNe 3

Last modified: We 31.07.2024 11:06