300306 UE Transmission Electron Microscopy: A hands-on course including immunolabelling of cells and tissues (2025W)
Hands-on Immunolabelling for Light and Electron Microscopy
Continuous assessment of course work
Labels
Laboratory and Field Methods
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 11.09.2025 14:00 to Th 25.09.2025 18:00
- Deregistration possible until We 15.10.2025 18:00
Details
max. 8 participants
Language: English
Lecturers
Classes (iCal) - next class is marked with N
The course language is English. The introduction to the course is on 14..11.25 and the practical course (two week practical block course) runs between 17.11.25 and 28.11.2025 inclusive . Please note, there is an additional meeting approx. 2 weeks after the final day of the block course, for the oral exams and also presentation of students poster or protocols. The last date for the final presentations will be discussed to suit all course-participating students.
Compulsory are all the lectures in Seminar room 1.8 (listed above), starting with Friday 14.11.2025 and also the excursion to ISTA campus on 28.11.2025. The practical sessions are designed to be modular in a 2 week block. To accommodate all students with needed essential equipment, each of 5 x half day practical modules will be offered twice. For assignment of students to sessions, more information will be given on Friday 14.11 and choices will be final on Monday 17.11.25.
If you have any further questions, please contact the course leader, jacqueline.montanaro(at)univie.ac.at
- Friday 14.11. 13:15 - 14:45 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
- Monday 17.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
- Wednesday 19.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
- Friday 21.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
- Monday 24.11. 08:00 - 09:30 Seminarraum 1.8, Biologie Djerassiplatz 1, 1.007, Ebene 1
- N Friday 19.12. 11:30 - 14:45 Seminarraum 1.2, Biologie Djerassiplatz 1, 1.004, Ebene 1
Information
Aims, contents and method of the course
The goal of the course is to teach Masters and PhD students about different methods which can be used to prepare and perform routine electron microscopy (EM), including how to prepare semi-thin and ultrathin sections using an ultramicrotome and an introduction to the transmission electron microscope. In addition, immunolabelling and contrasting using negative staining and acrylic sections will be evaluated and compared. Finally, stereological methods that may be useful for quantification of immunogold labelling will be presented and discussed. Advanced methods for sample preparation and other label detection methods will be discussed. Students participating in this course will gain a good understanding of routine and immunological methods for transmission EM. In addition, students can gain practical experience in preparing samples for electron microscopy and also how to interpret both routine preparations for transmission electron microscopy and how to interpret results of immunolabelling experiments.
Assessment and permitted materials
Each part must be positive:
Short presentation: 30 points
Detailed protocol or poster: 35 points
Oral examination: 35 points0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1
Short presentation: 30 points
Detailed protocol or poster: 35 points
Oral examination: 35 points0 - 50 points 5
51 - 67 points 4
67 - 81 points 3
82 - 90 points 2
91 - 100 points 1
Minimum requirements and assessment criteria
as above
Examination topics
Knowledge of the theoretical background (lectures); Safety in the electron microscopy lab. Understanding of different preparation methods. Applications of the technical aspects of immunolabeling for LM and TEM (from the practical part). Analysis, interpretation and evaluation of results.Detailed protocol or poster: 35 points
You are required to produce either a poster or protocol of your combined practicum efforts. It should include aims, methods, results and discussion. You will have questions from the examiners, observers and fellow students. You will be expected to demonstrate understanding of all techniques you performed in the class practicum, and be able to offer your own evaluation, interpretation and implications of the results.Short presentation (of your poster or protocol): 30 points
This is a short presentation (not longer than 7 mins) in the lecture room, presenting an aspect of your poster or protocol to the class. You will have questions from the examiners, observers and fellow students. You will be expected to demonstrate understanding of all techniques you performed in the class practicum, and be able to offer your own interpretation and implications of the results.Oral examination: 35 points : (option 1 or 2 chosen by class vote during practical time).
Option 1: about 2 weeks after the short presentation of your poster or protocol, you need to make a 10min video of yourself on your phone, answering a new exam question. You are expected to describe how to apply the techniques you learnt in the course (theory and practical material) to a new sample. You will have 1 week to prepare and upload. Points for completeness of answer, accurate timing (10-11 mins only). Longer presentations will reduce marks (eg. twice as long = 50% of mark). Short presentations are unlikely to cover material required. Please stick to question and topic in question.Option 2: the same oral exam question can be answered live and in public during class time, with examiner cold-calling on different students. Open class discussion. (approx. 30 min needed)
You are required to produce either a poster or protocol of your combined practicum efforts. It should include aims, methods, results and discussion. You will have questions from the examiners, observers and fellow students. You will be expected to demonstrate understanding of all techniques you performed in the class practicum, and be able to offer your own evaluation, interpretation and implications of the results.Short presentation (of your poster or protocol): 30 points
This is a short presentation (not longer than 7 mins) in the lecture room, presenting an aspect of your poster or protocol to the class. You will have questions from the examiners, observers and fellow students. You will be expected to demonstrate understanding of all techniques you performed in the class practicum, and be able to offer your own interpretation and implications of the results.Oral examination: 35 points : (option 1 or 2 chosen by class vote during practical time).
Option 1: about 2 weeks after the short presentation of your poster or protocol, you need to make a 10min video of yourself on your phone, answering a new exam question. You are expected to describe how to apply the techniques you learnt in the course (theory and practical material) to a new sample. You will have 1 week to prepare and upload. Points for completeness of answer, accurate timing (10-11 mins only). Longer presentations will reduce marks (eg. twice as long = 50% of mark). Short presentations are unlikely to cover material required. Please stick to question and topic in question.Option 2: the same oral exam question can be answered live and in public during class time, with examiner cold-calling on different students. Open class discussion. (approx. 30 min needed)
Reading list
Will be announced during the practical course
Association in the course directory
MZO4, MBO 7, MEC-9, MZO2, CoBeNe 3
Last modified: Fr 28.11.2025 12:07