300316 SE+UE Fluorescence in situ hybridization (FISH) (2005W)
Fluorescence in situ hybridization (FISH)
Continuous assessment of course work
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Vorbesprechung: 5.10.2005, 10:00 Uhr im HS II, Biozentrum. Block: 1.2. - 10.2.06, Übungsraum 7
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max. 12 participants
Language: German
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Last modified: Fr 31.08.2018 08:56
This laboratory course will teach attendants how to specifically detect and identify prokaryotes in environmental and medical samples using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.